Overexpression of plant uncoupling mitochondrial protein in transgenic tobacco increases tolerance to oxidative stress

dc.contributorUniversidade Estadual de Campinas (UNICAMP)
dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:50:09Z
dc.date.available2014-05-20T13:50:09Z
dc.date.created2014-05-20T13:50:09Z
dc.date.issued2003-06-01
dc.identifierJournal of Bioenergetics and Biomembranes. New York: Kluwer Academic/plenum Publ, v. 35, n. 3, p. 203-209, 2003.
dc.identifier0145-479X
dc.identifierhttp://hdl.handle.net/11449/17903
dc.identifier10.1023/A:1024603530043
dc.identifierWOS:000183994500002
dc.identifier8649222099176162
dc.description.abstractAn Arabidopsis thaliana cDNA clone encoding a plant uncoupling mitochondrial protein (AtPUMP1) was overexpressed in transgenic tobacco plants. Analysis of the AtPUMP1 mRNA content in the transgenic lines, determined by Northern blot, revealed variable levels of transgene expression. Antibody probing of Western blots of mitochondrial proteins from three independent transgenic lines showed significant accumulation of AtPUMP1 in this organelle. Overproduction of AtPUMP1 in transgenic tobacco plants led to a significant increase in tolerance to oxidative stress promoted by exogenous hydrogen peroxide as compared to wild-type control plants. These results provide the first biological evidence for a role of PUMP in protection of plant cells against oxidative stress damage.
dc.languageeng
dc.publisherKluwer Academic/plenum Publ
dc.relationJournal of Bioenergetics and Biomembranes
dc.relation2.914
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjecttransgenic tobacco
dc.subjectplant uncoupling protein
dc.subjectoxidative stress
dc.subjectconstitutive overexpression
dc.titleOverexpression of plant uncoupling mitochondrial protein in transgenic tobacco increases tolerance to oxidative stress
dc.typeArtículos de revistas


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