dc.contributor | Umea Univ | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.contributor | Univ Arkansas Med Sci | |
dc.contributor | Univ Gothenburg | |
dc.date.accessioned | 2014-05-20T13:45:59Z | |
dc.date.available | 2014-05-20T13:45:59Z | |
dc.date.created | 2014-05-20T13:45:59Z | |
dc.date.issued | 2012-01-01 | |
dc.identifier | Molecular Immunology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 49, n. 4, p. 601-610, 2012. | |
dc.identifier | 0161-5890 | |
dc.identifier | http://hdl.handle.net/11449/16233 | |
dc.identifier | 10.1016/j.molimm.2011.10.009 | |
dc.identifier | WOS:000300212100005 | |
dc.description.abstract | Cytokines produced by inflammatory or resident mesenchymal cells play important modulatory roles in the pathogenesis of inflammation induced bone loss. In the present study, the effects of IL-4 and IL-13 on the expression of three osteotropic cytokines in the IL-6 family expressed in human gingival fibroblasts were studied. IL-4R alpha and IL-13R alpha 1 mRNA were constitutively expressed in human gingival fibroblasts. The inflammatory cytokines IL-1 beta and TNF-alpha increased expression of IL-5, LIF, and IL-11 mRNA and protein in the gingival fibroblasts. Addition of IL-4 or IL-13 had no effect on IL-6 expression, but significantly inhibited LIF and IL-11 mRNA and protein stimulated by IL-1 beta and TNF-alpha. No involvement of NF-kappa B or STAT1 was observed in the inhibition. STAT6 was phosphorylated at Y641 by treatment with IL-4 and knockdown of STAT6 with siRNA decreased the inhibition of IL-11 and LIF expression by IL-4 in IL-1 beta and TNF-alpha stimulated cells. This study suggests that activation of STAT6 by IL-4 and IL-13, through type 2 IL-4 receptors, inhibits production of IL-11 and LIF stimulated by IL-1 beta and TNF-alpha in human gingival fibroblasts. A negative modulatory role of IL-4 and IL-13 in osteotropic cytokine production could be a mechanism playing an important inhibitory role in inflammation induced periodontitis. (C) 2011 Elsevier Ltd. All rights reserved. | |
dc.language | eng | |
dc.publisher | Pergamon-Elsevier B.V. Ltd | |
dc.relation | Molecular Immunology | |
dc.relation | 3.188 | |
dc.relation | 1,352 | |
dc.rights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | Interleukin-4 | |
dc.subject | Interleukin-6 | |
dc.subject | Gingival fibroblasts | |
dc.subject | Bone resorption | |
dc.title | Interleukin-4 and interleukin-13 inhibit the expression of leukemia inhibitory factor and interleukin-11 in fibroblasts | |
dc.type | Artículos de revistas | |