Artículos de revistas
Use of Adipose Tissue-Derived Mesenchymal Stem Cells for Experimental Tendinitis Therapy in Equines
Fecha
2011-01-01Registro en:
Journal of Equine Veterinary Science. New York: Elsevier B.V., v. 31, n. 1, p. 26-34, 2011.
0737-0806
10.1016/j.jevs.2010.11.014
WOS:000286447900005
WOS000286447900005.pdf
7773733250141398
9795829022108105
6020984937849801
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
Superficial digital flexor tendon lesion is an important cause of lameness in equine athletes. Although numerous treatments have been described, few are effective at promoting significant improvement in the quality, of the extracellular matrix. Therefore, great potential remains for recurrence and in certain cases, an abrupt end to the horse's athletic career. Recently, several experiments have focused on the therapeutic potential of mesenchymal stem cells (MSCs) in cases of tendon lesions. This study aimed to evaluate the effect of adipose tissue-derived MSCs in the treatment of induced tendinitis of the superficial digital flexor tendon in horses by clinical, ultrasonographic, histopathological, and immunochemical analyses. Tendinitis was induced in both thoracic limbs of eight mares by administration of collagenase solution and adipose tissue was collected from the tail base for MSCs isolation and expansion, which were used during cellular therapy on only one limb 30 days after lesion induction. No differences occurred between the groups regarding the clinical and ultrasonographic analyses; however, histopathological evaluation revealed a significant improvement in tendon fiber organization and diminished inflammatory infiltrate, whereas immunohistochemical analysis showed increased expression of type I collagen in the treated group as compared with controls. The cellular therapy model implanted in this experiment promoted increased perivascular inflammatory infiltrate, fibroblastic density, neovascularization, and qualitative healing improvement of tendon extracellular matrix, in terms of fiber orientation and type I/III collagen ratio; moreover, it was considered to be a safe and viable process. (C) 2011 Elsevier B.V. All rights reserved.