The heminested RT-PCR for the study of rabies virus pathogenesis

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:38:10Z
dc.date.available2014-05-20T13:38:10Z
dc.date.created2014-05-20T13:38:10Z
dc.date.issued2005-03-01
dc.identifierJournal of Virological Methods. Amsterdam: Elsevier B.V., v. 124, n. 1-2, p. 79-85, 2005.
dc.identifier0166-0934
dc.identifierhttp://hdl.handle.net/11449/13241
dc.identifier10.1016/j.jviromet.2004.11.008
dc.identifierWOS:000226883100011
dc.description.abstractThe aim of the present trial was to evaluate the heminested RT-PCR for the study of rabies virus distribution in mice inoculated experimentally. Inoculation was by the intramuscular route in 150 mice, using the dog street rabies virus. Groups of five animals were killed at different times. Fragments of different organs were collected and the material was tested by Fluorescent Antibody Test (FAT) and heminested RT-PCR (hn RT-PCR). Positive results were obtained beginning on the 10th day after inoculation in the brain, spinal cord, salivary gland, limbs, lungs, liver, spleen, urinary bladder, tongue and right kidney. Hn RT-PCR was shown to be more efficient for the study of rabies virus distribution in different tissues and organs. (C) 2004 Elsevier B.V.. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationJournal of Virological Methods
dc.relation1.756
dc.relation0,858
dc.rightsAcesso restrito
dc.sourceWeb of Science
dc.subjectFluorescent Antibody Test (FAT)
dc.subjecthn RT-PCR
dc.subjectrabies
dc.subjectpathogenesis
dc.titleThe heminested RT-PCR for the study of rabies virus pathogenesis
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución