dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:32Z
dc.date.available2014-05-20T13:24:32Z
dc.date.created2014-05-20T13:24:32Z
dc.date.issued2003-06-01
dc.identifierBiological & Pharmaceutical Bulletin. Tokyo: Pharmaceutical Soc Japan, v. 26, n. 6, p. 905-908, 2003.
dc.identifier0918-6158
dc.identifierhttp://hdl.handle.net/11449/7645
dc.identifier10.1248/bpb.26.905
dc.identifierWOS:000183214700032
dc.identifierWOS000183214700032.pdf
dc.identifier7927877224326837
dc.identifier0000-0003-3032-2556
dc.description.abstractPaepalantine (9,10-dihydroxy-5,7-dimethoxy-1H-naphto(2,3c)pyran-1-one), a natural isocoumarin isolated from the capitula of Paepalanthus bromelioides (Eriocaulaceae), was assessed for its effect on the respiratory burst (zymosan-stimulated luminol-enhanced chemiluminescence and. PMA-stimulated lucigenin-enhanced chemiluminescence) of polymorphonuclear neutrophils in vitro. Special attention was devoted to establishing the IC50 for neutrophils. Paepalantine was able to decrease luminol and lucigenin chemiluminescence, reflecting an inhibitory effect on the respiratory burst, with an ED50 of 0.44 +/- 0.05 and 0.84 +/- 0.15 mug/ml, respectively. A cell-free system was performed with paepalantine on myeloperoxidase/H2O2 and myeloperoxidase/H2O2/Cl- systems. Paepalantine inhibited luminol oxidation in both systems. This inhibition was related to the interaction of paepalantine-myeloperoxidase and its scavenger effect on HOCl.
dc.languageeng
dc.publisherPharmaceutical Soc Japan
dc.relationBiological & Pharmaceutical Bulletin
dc.relation1.694
dc.relation0,626
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.subjectantioxidant activity
dc.subjectpaepalantine
dc.subjectisocoumarin
dc.subjectchemiluminescence
dc.titleEffect of the isocoumarin paepalantine on the luminol and lucigenin amplified chemiluminescence of rat neutrophils
dc.typeArtículos de revistas


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