Artículos de revistas
Prevalence and Molecular Characterization of Mycoplasma ovis in Selected Free-Ranging Brazilian Deer Populations
Fecha
2011-10-01Registro en:
Journal of Wildlife Diseases. Lawrence: Wildlife Disease Assoc, Inc, v. 47, n. 4, p. 1005-1011, 2011.
0090-3558
WOS:000296409500025
Autor
Purdue Univ
Universidade Federal do Paraná (UFPR)
Universidade Estadual Paulista (Unesp)
Universidade Federal de Uberlândia (UFU)
Universidade Estadual de Londrina (UEL)
Institución
Resumen
Mycoplasma ovis is a hemoplasma that may cause anemia and mortality in small ruminants. Our aim was to determine whether M. ovis infects populations of free-ranging deer in Brazil. Bully coat samples from 64 Blastocerus dichotomus from Porto Primavera, 18 Ozotocerus bezoarticus from Pantanal, and 21 O. bezoarticus from Emas National Park were tested. Using a M. ovis PCR protocol to amplify extracted DNA, 46/64 (72%) of deer froth Porto Primavera, 10/18 (56%) from Pantanal, and 4/21 (19%) from Emas National Park were positive, giving an overall positive rate of 58% for hemoplasma in these wild deer. Sequencing and phylogenetic analysis of the 168 rRNA gene revealed 3 genetically distinct hemoplasmas including M. ovis, 'Candidatus Mycoplasma erythrocervae', and a hemoplasma most closely related to M. ovis. Phylogenetic analysis of the 23S rRNA gene from selected sequences confirmed these relationships.