Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa

dc.contributorUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:15:55Z
dc.date.available2014-05-20T13:15:55Z
dc.date.created2014-05-20T13:15:55Z
dc.date.issued2006-03-01
dc.identifierRevista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 8, n. 1, p. 51-54, 2006.
dc.identifier1516-635X
dc.identifierhttp://hdl.handle.net/11449/2927
dc.identifier10.1590/S1516-635X2006000100008
dc.identifierS1516-635X2006000100008
dc.identifierS1516-635X2006000100008.pdf
dc.description.abstractThis study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
dc.languageeng
dc.publisherFundação APINCO de Ciência e Tecnologia Avícolas
dc.relationRevista Brasileira de Ciência Avícola
dc.relation0.463
dc.rightsAcesso aberto
dc.sourceSciELO
dc.subjectChicken
dc.subjectELISA
dc.subjectenzyme
dc.subjectSalmonella sp
dc.subjectSerology
dc.titleChicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución