dc.contributorUniversidade Estadual Paulista (Unesp)
dc.contributorUniversidade Federal de Uberlândia (UFU)
dc.contributorUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:15:29Z
dc.date.available2014-05-20T13:15:29Z
dc.date.created2014-05-20T13:15:29Z
dc.date.issued2011-09-01
dc.identifierJournal of Veterinary Science. Seoul: Korean Soc Veterinary Science, v. 12, n. 3, p. 267-272, 2011.
dc.identifier1229-845X
dc.identifierhttp://hdl.handle.net/11449/2614
dc.identifier10.4142/jvs.2011.12.3.267
dc.identifierWOS:000295017500010
dc.identifierWOS000295017500010.pdf
dc.description.abstractThe objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three ejaculates per bull were evaluated. Frozen semen samples were thawed and the acrosomal region of sperm cells was evaluated by transmission electron microscopy (TEM) before (n = 18) and after (n = 18) Percoll centrifugation. The evaluation of 20 sperm heads from each of the 36 samples analyzed ensured that a large number of cells were investigated. The data were subjected to analysis of variance at a level of significance of 5%. Percoll centrifugation reduced the percentage of sperm exhibiting normal acrosomes (from 61.77 to 30.24%), reduced the percentage of sperm presenting atypical acrosome reactions (from 28.38 to 4.84%) and increased the percentage of sperm exhibiting damage in the acrosome (from 6.14 to 64.26%). The percentage of sperm with typical acrosome reactions was not significantly different before (3.70%) and after (0.67%) centrifugation. TEM distinguished four different types of acrosomal status and enabled ultrastructural characterization of acrosomal injuries. The percentage of sperm exhibiting normal acrosomes decreased and damage in the acrosome was the most frequent acrosomal injury with the Percoll gradient centrifugation protocol utilized.
dc.languageeng
dc.publisherKorean Society Veterinary Science
dc.relationJournal of Veterinary Science
dc.relation1.327
dc.rightsAcesso aberto
dc.sourceWeb of Science
dc.subjectacrosomal ultrastructure
dc.subjectBovine
dc.subjectPercoll
dc.subjectsperm selection
dc.titleTransmission electron microscopy for characterization of acrosomal damage after Percoll gradient centrifugation of cryopreserved bovine spermatozoa
dc.typeArtículos de revistas


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