Artículos de revistas
Effect of a recombinant Lys49 PLA2 myotoxin and Lys49 PLA2-derived synthetic peptides from Agkistrodon species on membrane permeability to water.
Fecha
2004Registro en:
0041-0101
10.1016/j.toxicon.2004.05.013
15284020
Autor
Leite, R. S.
Giuliani, C. D.
Lomonte, Bruno
Franco, Wilfredo
Selistre de Araujo, Heloisa S.
Institución
Resumen
The aim of this work was to study the effect of recombinant ACL myotoxin, a Lys49PLA2 from Agkistrodon contortrix
laticinctus snake venom and Lys49PLA2-derived synthetic peptides corresponding to the region 115–129 of venom of the two
different Agkistrodon species on water permeability in the toad urinary bladder. The water flow through the membrane was
measured gravimetrically in bag preparations of the bladder. The addition of recombinant ACL myotoxin-MBP (maltose
binding protein) fusion protein (10 nM) to the bathing solution significantly increased (above 60%) the water transport
compared with the control hemibladders. The addition of the Lys49PLA2-derived synthetic peptides in several concentrations
to the bathing solution did not affect the water transport across membrane. These results suggest that the ACL myotoxin effect
on water transport is not related to the cytotoxic C-terminal region.