Thidiazuron-induced shoot organogenesis of Cleome viscosa (L) through cotyledonary explants culture

dc.creatorENRIQUE CASTAÑO DE LA SERNA
dc.date2014-02-26
dc.date.accessioned2018-11-19T14:14:54Z
dc.date.available2018-11-19T14:14:54Z
dc.identifierhttp://cicy.repositorioinstitucional.mx/jspui/handle/1003/724
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/2256450
dc.descriptionA reproducible protocol for direct shoot organogenesis of Cleome viscosa, an important medicinal weed herb was developed. The seed explants were collected primarily from field grown mature plants for in vitro germination on different strength of MS basal medium with or without selection of GA3 at various concentration (0.1 to 1.0 mg/L). The highest rate of seed germination (55.3%) was noticed on full strength MS basal medium fortified with 0.5 mg/L GA3 after 30 days of culture. The excised 7 to 10 days old cotyledonary leaf, cotyledonary node and hypocotyls explants cultured on MS medium fortified with different concentration of individual cytokinin (BA/KIN/TDZ) alone or BA+KIN or TDZ+KIN or TDZ in combinations with different auxins (IBA/NAA/IAA) influenced the frequency of adventitious microshoot formation.
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/dataset/DOI/DOI: 10.5897/AJB2013.12456
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceAfrican Journal of Biotechnology. 13(9), 2014
dc.subjectinfo:eu-repo/classification/Autores/CLEOME VISCOSA L
dc.subjectinfo:eu-repo/classification/Autores/MEDICINAL WEED PLANT
dc.subjectinfo:eu-repo/classification/Autores/SHOOT MULTIPLICATION
dc.subjectinfo:eu-repo/classification/Autores/DIRECT ORGANOGENESIS
dc.subjectinfo:eu-repo/classification/cti/2
dc.titleThidiazuron-induced shoot organogenesis of Cleome viscosa (L) through cotyledonary explants culture
dc.typeArtículos de revistas
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución