Artículos de revistas
Influence of form of activated charcoal on embryogenic callus formation in coconut (Cocos nucifera)
Autor
LUIS ALFONSO SAENZ CARBONELL
Institución
Resumen
Development of micropropagation protocols for
Cocos nucifera has progressed slowly. Activated charcoal is
included in the culture medium of each protocol, mainly to
prevent tissue browning. Charcoal production procedures
can affect the properties of different brands. In this study,
eight types of activated charcoal were evaluated for their
effects on free 2,4-dichlorophenoxyacetic acid level, pH,
conductivity, and osmolarity of the culture medium and on
the frequency of embryogenic callus induction. Moreover,
the effect of particle size of the optimum charcoal type on
embryogenic callus development was also studied. Charcoal
type had a significant effect on (Y3) culture medium properties.
Free 2,4-D was highest in Reactivos y Productos
Quı
´
micos Finos-containing medium and pH was lowest in
MERCK-containing medium. Charcoal type also influenced
embryogenic callus induction, with acid washed for plant
cell and tissue culture-, DARCO- and United States Pharmacopeia-containing
media promoting *60% embryogenic
callus, but with different optimal 2,4-D concentrations.
Particle size profiles varied among all charcoal types,
although small particle fraction (\38 lm) was abundant in
all. Use of small particle fractions produced higher frequencies
of embryogenic callus (70%) than either large
particle or whole charcoal fractions.