| dc.creator | Oscar Moreno_Valenzuela | |
| dc.date | 2013-04-02 | |
| dc.date.accessioned | 2018-11-19T14:12:32Z | |
| dc.date.available | 2018-11-19T14:12:32Z | |
| dc.identifier | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/21 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2255987 | |
| dc.description | Virus-induced gene silencing is based on
the sequence-specific degradation of RNA. Here, a
gene silencing vector derived from EuMV-YP, named
pEuMV-YP:DAV1, was used to silence ChlI and
NPR1 genes in Nicotiana benthamiana. The silencing
of the ChlI transcripts was efficient in the stems,
petioles and leaves as reflected in tissue bleaching and
reduced transcript levels. The silencing was stable,
reaching the flowers and fruits, and was observed
throughout the life cycle of the plants. Additionally,
the silencing of the NPR1 gene was efficient in both
N. benthamiana and Capsicum annuum. After silencing,
the plants’ viral symptoms increased to levels
similar to those seen in wild-type plants. These results
suggest that NPR1 plays a role in the compatible
interactions of EuMV-YP N. benthamiana and
EuMV-C. annum var. anaheim | |
| dc.format | application/pdf | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.subject | info:eu-repo/classification/cti/2 | |
| dc.title | A new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim | |
| dc.type | Artículos de revistas | |
