| dc.creator | Sanghamitra Khandual | |
| dc.creator | FLOR YOHANA FLORES HERNANDEZ | |
| dc.creator | INOCENCIA GUADALUPE RAMIREZ LOPEZ | |
| dc.date | 2016-11-23 | |
| dc.date.accessioned | 2018-11-19T14:00:07Z | |
| dc.date.available | 2018-11-19T14:00:07Z | |
| dc.identifier | http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/390 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2253776 | |
| dc.description | "Objective: To evaluate the cytotoxic effects of Spirulina platensis extracts on acute
leukemia Kasumi-1 and chronic leukemia K-562 cancer cell lines.
Methods: Various concentrations of Spirulina platensis extracts (0.25–50.00 mg/mL)
obtained with different solvents were used to treat cell lines for 72 h. For cytotoxic effect
studies, cell viability test with trypan blue solution, MTT assay and microscopic cytomorphological
assessment were done.
Results: Spirulina extract obtained with 70% ethanol showed significant cytotoxicity in
K562 and Kasumi-1 cell lines. With trypan blue solution, IC50 values were found to be
4.64 mg/mL for K-562 and 3.68 mg/mL for Kusumi-1 cell lines. Spirulina aqueous
extract also showed cytotoxicity with trypan blue method, at a slightly higher dose; where
IC50 values were 12.68 mg/mL for K-562 and 2.13 mg/mL for Kusumi-1 cell lines. The
IC50 values were found 0.40 mg/mL for K-562 and 0.31 mg/mL for Kusumi-1 cell lines
for the 70% ethanol extract according to the MTT assay. Spirulina extract obtained with
water also showed cytotoxicity but the dose was a little higher where IC50 values were
15.77 mg/mL for K-562 and 9.44 mg/mL for Kusumi-1 cell lines. The effect of cytotoxicity
with ethanol extract is quite comparable with that observed for cyclophosphamide,
which is a chemical used as anticancer agent.
Conclusions: The cytotoxicity exhibited by Spirulina extract to cancer cell lines might
be due to the presence of phytopigments (carotenoids, chlorophyll, phycocyanin) as well
as polysaccharides that were reported previously as constituents of the extract. So crude
extracts of Spirulina can be used as a source to develop anticancer drugs". | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation | info:eu-repo/semantics/dataset/URL/http://www.sciencedirect.com/science/article/pii/S2221169116310875?via%3Dihub | |
| dc.relation | info:eu-repo/semantics/dataset/DOI/https://doi.org/10.1016/j.apjtb.2016.10.011 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.subject | info:eu-repo/classification/Autor/Leukemia, Cytotoxicity, Spirulina, Carotenoids | |
| dc.subject | info:eu-repo/classification/cti/3 | |
| dc.subject | info:eu-repo/classification/cti/32 | |
| dc.subject | info:eu-repo/classification/cti/2410 | |
| dc.subject | info:eu-repo/classification/cti/241003 | |
| dc.title | Cytotoxic effect of Spirulina platensis extracts on human acute leukemia Kasumi-1 and chronic myelogenous leukemia K-562 cell lines. | |
| dc.type | Artículos de revistas | |
| dc.type | Artículos de revistas | |
| dc.audience | students | |
| dc.audience | researchers | |
