| dc.creator | Sanghamitra Khandual | |
| dc.date | 2014-03 | |
| dc.date.accessioned | 2018-11-19T13:58:20Z | |
| dc.date.available | 2018-11-19T13:58:20Z | |
| dc.identifier | http://ciatej.repositorioinstitucional.mx/jspui/handle/1023/111 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2253448 | |
| dc.description | A rapid, efficient and high-performance transformation protocol employing Agrobacterium rhizogenes
was developed for the common bean Phaseolus vulgaris. In this study, we examined competencies
of various protocols to induce and explants that respond to hairy root transformation in
bean plants. Utilizing young seedlings with severed radicles/hypocotyls, we developed a highly efficient
procedure for achieving hairy root transformation frequencies as high as 100% as visualized
by GUS reporter gene expression system. Transgenic hairy roots in these young composite
plants were susceptible to nodulation by rhizobia, and form an excellent system for high throughput
genomic analysis to study root biology and endosymbiosis in common bean. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | SCIENTIFIC RESEARCH | |
| dc.relation | info:eu-repo/semantics/dataset/DOI/http://file.scirp.org/pdf/ABB_2014031213234139.pdf | |
| dc.relation | citation:Khandual, S., & Reddy, P. M. (2014). Rapid, Efficient and High-Performance Protocol for Agrobacterium rhizogenes-Mediated Hairy Root Transformation of the Common Bean Phaseolus vulgaris. Advances in Bioscience and Biotechnology, 2014. | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.subject | info:eu-repo/classification/AUTOR/Phaseolus vulgaris; Agrobacterium rhizogenes; Hairy Root Transformation; GUS Expression | |
| dc.subject | info:eu-repo/classification/cti/6 | |
| dc.title | Rapid, Efficient And High-Performance Protocol For Agrobacterium Rhizogenes-Mediated Hairy Root Transformation Of The Common Bean Phaseolus Vulgaris. | |
| dc.type | Artículos de revistas | |
| dc.audience | students | |
| dc.audience | researchers | |
