"The research work presented in this thesis shows a successful and promising application of an optical non destructive testing technique known as Digital Holographic Interferometry (DHI). DHI is applied to detect and obtain deformations from butterflies’ wings during flapping motion; this generates displacement maps for the whole wings surface. The in-vivo tests and their corresponding results were recorded for five different winged butterfly species. The first part of this research work, presents the measurement performed on wings from a Pterourus Multicaudata known as Eastern Swallow Tiger Butterfly, as a proof of principle that fast non repeatable events on organic tissues can be detected with high accuracy using DHI. The second part of this work presents a comparison from four different specimens, namely Nymphalis Antiopa known as Mourning Cloak, Agraulis Vanillae Incarnata known as Gulf fritillary, Danaus Gilippus Cramer and Precis Evarete Felder known as Queen Butterfly and Buckeye Butterfly respectively. All of them have a different structure and wing shape. The DHI optical set up used has an out of plane sensibility that enables to measure changes perpendicular to the wings surface with hundreds of nanometers resolution. The wings motion is recorded with a high speed camera at rates of 500 frames per second for the proof of principle test, and 4000 frames per second for the remaining tests. The camera uses CMOS technology with 800 x 800 pixels resolution and dynamic range of 10 bits; features that allow the detection of small changes during wings flapping. The wing deformation maps are presented in a pseudo 3D representation, and they show the relative deformation of the insect’s wing for a fraction of time during the up and down stroke movements. A time vs. displacement tracking plot of randomly chosen wing sections is presented to show the differences of behaviour among different wing sections of a butterfly during normal flapping."