Phylogenetic diversity of methyl-coenzyme M reductase (mcrA) gene and methanogenesis from trimethylamine in hypersaline environments

dc.creatorJOSE QUINATZIN GARCIA MALDONADO
dc.creatorMARIA DE LOURDES BERENICE CELIS GARCIA
dc.creatorALEJANDRO LOPEZ CORTES
dc.date2012
dc.date.accessioned2018-11-19T13:52:18Z
dc.date.available2018-11-19T13:52:18Z
dc.identifierhttp://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1244
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/2252307
dc.description"Methanogens have been reported in complex microbial communities from hypersaline environments, but little is known about their phylogenetic diversity. In this work, methane concentrations in environmental gas samples were determined while methane production rates were measured in microcosm experiments with competitive and non-competitive substrates. In addition, the phylogenetic diversity of methanogens in microbial mats from two geographical locations was analyzed: the well studied Guerrero Negro hypersaline ecosystem, and a site not previously investigated, namely Laguna San Ignacio, Baja California Sur, Mexico. Methanogenesis in these microbial mats was suspected based on the detection of methane (in the range of 0.00086 to 3.204 %) in environmental gas samples. Microcosm experiments confirmed methane production by the mats and demonstrated that it was promoted only by non-competitive substrates (trimethylamine and methanol), suggesting that methylotrophy is the main characteristic process by which these hypersaline microbial mats produce methane. Phylogenetic analysis of amino acid sequences of the methyl coenzyme-M reductase (mcrA) gene from natural and manipulated samples revealed various methylotrophic methanogens belonging exclusively to the family Methanosarcinaceae. Moderately halophilic microorganisms of the genus Methanohalophilus were predominant (>60 % of mcrA sequences retrieved). Slightly halophilic and marine microorganisms of the genera Methanococcoides and Methanolobus, respectively, were also identified, but in lower abundances."
dc.formatapplication/pdf
dc.languageeng
dc.relationinfo:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.2436/20.1501.01.155
dc.relationcitation:García-Maldonado JQ, Bebout BM, Celis LB & López-Cortés A.. (2012). Phylogenetic diversity of methyl-coenzyme M reductase (mcrA) gene and methanogenesis from trimethylamine in hypersaline environments.. International Microbiology, 15, 33-41 https://doi.org/10.2436/20.1501.01.155
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectinfo:eu-repo/classification/Autor/Methanosarcinaceae
dc.subjectinfo:eu-repo/classification/Autor/Hypersaline environments
dc.subjectinfo:eu-repo/classification/Autor/Microbial mats
dc.subjectinfo:eu-repo/classification/Autor/Trimethylamine
dc.subjectinfo:eu-repo/classification/Autor/Gene mcrA
dc.subjectinfo:eu-repo/classification/cti/6
dc.titlePhylogenetic diversity of methyl-coenzyme M reductase (mcrA) gene and methanogenesis from trimethylamine in hypersaline environments
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.audiencegeneralPublic


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