A germin-like protein gene (CchGLP) of Capsicum chinense Jacq. is induced during incompatible interactions and displays Mn-superoxide dismutase activity

dc.creatorMA. FABIOLA LEON GALVAN
dc.creatorAHUITZOLT DE JESUS JOAQUIN RAMOS
dc.creatorIRINEO TORRES PACHECO
dc.creatorANA PAULINA BARBA DE LA ROSA
dc.creatorLORENZO GUEVARA OLVERA
dc.creatorMARIO MARTIN GONZALEZ CHAVIRA
dc.creatorROSALIA VIRGINIA OCAMPO VELAZQUEZ
dc.creatorENRIQUE RICO GARCIA
dc.creatorRAMON GERARDO GUEVARA GONZALEZ
dc.date2011-10
dc.date.accessioned2018-11-19T13:52:11Z
dc.date.available2018-11-19T13:52:11Z
dc.identifierhttp://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1218
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/2252281
dc.description"A germin-like gene (CchGLP) cloned from geminivirus-resistant pepper (Capsicum chinense Jacq. Line BG-3821) was characterized and the enzymatic activity of the expressed protein analyzed. The predicted protein consists of 203 amino acids, similar to other germin-like proteins. A highly conserved cupin domain and typical germin boxes, one of them containing three histidines and one glutamate, are also present in CchGLP. A signal peptide was predicted in the first 18 N-terminal amino acids, as well as one putative N-glycosylation site from residues 44-47. CchGLP was expressed in E. coli and the recombinant protein displayed manganese superoxide dismutase (Mn-SOD) activity. Molecular analysis showed that CchGLP is present in one copy in the C. chinense Jacq. genome and was induced in plants by ethylene (Et) and salicylic acid (SA) but not jasmonic acid (JA) applications in the absence of pathogens. Meanwhile, incompatible interactions with either Pepper golden mosaic virus (PepGMV) or Pepper huasteco yellow vein virus (PHYVV) caused local and systemic CchGLP induction in these geminivirus-resistant plants, but not in a susceptible accession. Compatible interactions with PHYVV, PepGMV and oomycete Phytophthora capsici did not induce CchGLP expression. Thus, these results indicate that CchGLP encodes a Mn-SOD, which is induced in the C. chinense geminivirus-resistant line BG-3821, likely using SA and Et signaling pathways during incompatible interactions with geminiviruses PepGMV and PHYVV"
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI AG
dc.relationinfo:eu-repo/semantics/altIdentifier/DOI/http://doi.org/10.3390/ijms12117301
dc.relationcitation:León-Galván, F., de Jesús Joaquín-Ramos, A., Torres-Pacheco, I., Barba de la Rosa, A. P., Guevara-Olvera, L., González-Chavira, M. M., … Guevara-González, R. G. (2011). A Germin-Like Protein Gene (CchGLP) of Capsicum chinense Jacq. Is Induced during Incompatible Interactions and Displays Mn-Superoxide Dismutase Activity. International Journal of Molecular Sciences, 12(11), 7301–7313.
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectinfo:eu-repo/classification/Autor/GLP
dc.subjectinfo:eu-repo/classification/Autor/Geminiviruses
dc.subjectinfo:eu-repo/classification/Autor/Mn-SOD
dc.subjectinfo:eu-repo/classification/Autor/Ethylene
dc.subjectinfo:eu-repo/classification/Autor/Salicylic acid
dc.subjectinfo:eu-repo/classification/cti/2
dc.titleA germin-like protein gene (CchGLP) of Capsicum chinense Jacq. is induced during incompatible interactions and displays Mn-superoxide dismutase activity
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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