| dc.creator | LAURA DENNISSE CARRAZCO PEÑA | |
| dc.creator | ANA PAULINA BARBA DE LA ROSA | |
| dc.date | 2010-08 | |
| dc.date.accessioned | 2018-11-19T13:52:06Z | |
| dc.date.available | 2018-11-19T13:52:06Z | |
| dc.identifier | http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1199 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2252263 | |
| dc.description | "11S globulin is one of the major seed storage proteins in amaranth. Recombinant protein was produced as up to similar to 80% of the total bacterial protein using Escherichia coli Rosetta-gami (DE3) containing pET21d with amaranth 11S globulin cDNA. The best expression condition was at 302 K for 20 h using LB medium containing 0.5 M NaCl. The recombinant protein was easily separated from most of the Escherichia coli proteins by precipitation with 0-40% ammonium sulfate solution. It formed aggregates at low temperature and at low salt concentrations. This behaviour may imply that it has a more hydrophobic nature than other 11S seed globulins. The crystals diffracted to 6 A resolution and belonged to space group P6(3), with unit-cell parameters a = b = 97.6, c = 74.8 A, gamma = 120.0 degrees. One subunit of a trimer was estimated to be present in the asymmetric unit, assuming a V (sol) of 41%. To obtain the complete structure solution, experiments to improve crystallization and flash-cooling conditions are in progress." | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | International Union of Crystallography | |
| dc.relation | info:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1107/S1744309110021032 | |
| dc.relation | citation:Tandang-Silvas, M. R., Carrazco-Pena, L., Barba de la Rosa, A. P., Osuna-Castro, J. A., Utsumi, S., Mikami, B. & Maruyama, N. (2010). Acta Cryst. F66, 919-922. | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.subject | info:eu-repo/classification/Autor/Seed storage proteins | |
| dc.subject | info:eu-repo/classification/Autor/Amaranthus hypochondriacus L. | |
| dc.subject | info:eu-repo/classification/Autor/Amaranth 11S proglobulin | |
| dc.subject | info:eu-repo/classification/cti/2 | |
| dc.title | Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L. | |
| dc.type | Artículos de revistas | |
| dc.type | Artículos de revistas | |
