| dc.creator | MA. FABIOLA LEON GALVAN | |
| dc.creator | IRINEO TORRES PACHECO | |
| dc.creator | LORENZO GUEVARA OLVERA | |
| dc.creator | ANA PAULINA BARBA DE LA ROSA | |
| dc.creator | MARIO MARTIN GONZALEZ CHAVIRA | |
| dc.creator | RAMON GERARDO GUEVARA GONZALEZ | |
| dc.date | 2010-12 | |
| dc.date.accessioned | 2018-11-19T13:51:55Z | |
| dc.date.available | 2018-11-19T13:51:55Z | |
| dc.identifier | http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1165 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2252250 | |
| dc.description | "Microbial tannase, a hydrolysable tannin-degrading enzyme, is extensively used in manufacture of instant tea, beer, wine, and gallic acid. Aspergillus niger strain, obtained from a Mexican tannery wastewaters rich in gallic acid [Quebracho Phenolics-rich Tannery Wastewaters, (QPTW)], displayed a good growth and tannase activity in a minimal medium added with 1% (w/v) QPTW (Kr= 0.451 mm.h-1). Using PCR and RACE 3´ and 5´methodologies, a complete cDNA of a tannase was cloned from this isolate.Nucleotide sequence of complete cDNA was of 4690 bp with a complete ORF of 1833 bp encoding 611 amino acids. Transcriptionalinduction was observed in mineral medium added with carbon sources as tannic acid alone (1 and 10 g/l), as well as mix of glucose(1 and 10 g/l) and tannic acid (1 g/l) in the media. However, neither glucose (1 and 10 g/l) and sucrose (1 and 10 g/l) nor (+)-catechin(1 and 10 g/l) as sole carbon sources displayed gene induction in in vitro assays. A. niger-GTO is a new strain with interesting characteristics for industrial tannase production purposes." | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | National Institute of Science Communication And Information Resources | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.subject | info:eu-repo/classification/Autor/Tannase | |
| dc.subject | info:eu-repo/classification/Autor/Aspergillus niger | |
| dc.subject | info:eu-repo/classification/Autor/Guanajuato isolate | |
| dc.subject | info:eu-repo/classification/Autor/cDNA cloning | |
| dc.subject | info:eu-repo/classification/cti/7 | |
| dc.title | Molecular and biochemical characterization of extracellular tannin acyl hydrolase activity from a Mexican isolate of Aspergillus niger | |
| dc.type | Artículos de revistas | |
| dc.type | Artículos de revistas | |
| dc.audience | generalPublic | |
