Uso de oócitos bovinos como citoplasma receptor na produção de embriões por transferência nuclear de célula somática interespécie (NTSCi)

dc.contributorCNPqpt-BR
dc.creatorForell, Fabiana
dc.creatorFeltrin, Cristiano
dc.creatorSantos, Lucila Carboneiro dos
dc.creatorCosta, Ubirajara Maciel da
dc.creatorVieira, Arnaldo Diniz
dc.creatorHölker, Michael
dc.creatorRodrigues, José Luiz
dc.date2018-03-30
dc.date.accessioned2018-11-07T22:09:50Z
dc.date.available2018-11-07T22:09:50Z
dc.identifierhttps://seer.ufrgs.br/ActaScientiaeVeterinariae/article/view/17276
dc.identifier10.22456/1679-9216.17276
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/2193365
dc.descriptionInterspecies embryo clones have been produced by research groups with relative success in some species. Bovine oocytes matured in vitro and enucleated by micromanipulation were used in three experiments as recipeient cytoplasm in nuclear transfer of ovine, caprine and porcine fibroblasts. The fibroblasts were cultivated until the third passage before being frozen and used. The electrofusion was induced by an application of a 20V pulse during 45 ms. The activation was done with 5 mM ionomycin and subsequently 2 mM 6DMAP. NTSC bovine embryos, NTSCi caprine and ovine embryos were cultivated in SOF medium and NTSCi porcine embryos were cultivated in NCSU23 medium. The fusion rates of the reconstructed complexes with bovine cells did not differ from those observed with ovine cells (88.2%), caprine cells (74.1%) and porcine cells (79.4%). The cleavage rates in ovine (60.3%), caprine (68.4%) and porcine (57.1%) NTSCi groups did not differ from the control group NTSC bovine. The blastocyst rate observed in the group of NTSCi ovine embryos (10.3%) was similar to the group of NTSC bovine embryos (12.7%). In NTSCi caprine embryos, 5.3% of the embryos developed up to the blastocyst stage, while in the NTSCi porcine group there was no development up to the blastocyst stage. In conclusion, the bovine cytoplasm was able to support the embryo development in NTSCi up to the blastocyst stage using ovine and caprine fibroblasts as donor cells.pt-BR
dc.formatapplication/pdf
dc.languagepor
dc.publisherUniversidade Federal do Rio Grande do Sulen-US
dc.relationhttps://seer.ufrgs.br/ActaScientiaeVeterinariae/article/view/17276/10178
dc.rightsDireitos autorais 2018 Fabiana Forell, Cristiano Feltrin, Lucila Carboneiro dos Santos, Ubirajara Maciel da Costa, Arnaldo Diniz Vieira, Michael Hölker, José Luiz Rodriguespt-BR
dc.rightshttp://creativecommons.org/licenses/by/4.0pt-BR
dc.sourceActa Scientiae Veterinariae; v. 36, n. 2 (2008); 141-147en-US
dc.sourceActa Scientiae Veterinariae; v. 36, n. 2 (2008); 141-147pt-BR
dc.source1679-9216
dc.subjectReprodução Animalpt-BR
dc.subjectEmbriões; Clones; Interespécie; Transferência nuclear; In vitropt-BR
dc.titleUso de oócitos bovinos como citoplasma receptor na produção de embriões por transferência nuclear de célula somática interespécie (NTSCi)pt-BR
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.coveragept-BR
dc.coveragept-BR
dc.coveragept-BR


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