This research aimed to assess students’ perceptions regarding a multimedialearning laboratory, after experiencing biology classes that used a different teaching approach. The survey involved 159 students between 15 and 17 years old. The methodology combined quantitative and qualitative approaches and obtained results by using a questionnaire and by conducting interviews, respectively. Results indicate that students have good perceptions about the laboratory. They consider it as relevant, authentic, challenging, and useful, with fun and easy-to-use resources. They also point out that it provides opportunities for reflection about their own learning. Results further indicate that boys and girls have similar perceptions about the laboratory, and that students from different years of secondary education do not follow a pattern in their preferences.