Optimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic test

dc.creatorRozales, Franciéli Pedrotti
dc.creatorde-Paris, Fernada
dc.creatorMachado, Alice Beatriz Mombach Pinheiro
dc.creatorCosti, Cintia
dc.creatorBarth, Afonso Luis
dc.date2014-07-03
dc.identifierhttps://seer.ufrgs.br/index.php/hcpa/article/view/46502
dc.descriptionInfection with hepatitis C virus (HCV) is a global public health issue. The bloodborne nature of HCV transmission poses a substantial risk to healthcare workers, due to occupational exposure to needlestick injuries and blood and other body fluids containing the virus. Undiagnosed HCV infection, including in healthcare workers, represents a growing problem worldwide as the infected population ages, and HCV-related mortality and morbidity is expected to rise substantially over the coming decades. Consequently, diagnostic tests for HCV play an important role in this scenario. The aim of this study was to standardize a one-step RT-PCR assay for detection of HCV. The test demonstrated reproducibility, sensibility (100%), and the limit of detection was set at 100IU/mL. Our study indicates that this assay can be used as a diagnostic tool to follow up healthcare workers after occupational exposure. en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherHCPA/FAMED/UFRGSpt-BR
dc.relationhttps://seer.ufrgs.br/index.php/hcpa/article/view/46502/30860
dc.sourceClinical & Biomedical Research; Vol. 34 No. 2 (2014)en-US
dc.sourceClinical and Biomedical Research; v. 34 n. 2 (2014)pt-BR
dc.source2357-9730
dc.subjectHCVen-US
dc.subjectDiagnostic testen-US
dc.subjectX-tailen-US
dc.subjectAcute diseaseen-US
dc.titleOptimization of one-step real-time PCR for the X-tail target of HCV as a diagnostic testen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
dc.typeAvaliado por Parespt-BR


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