info:eu-repo/semantics/article
Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases
Fecha
2014-01Registro en:
Jaureguiberry, María Soledad; Tricerri, Maria Alejandra; Sanchez, Susana A.; Finarelli, Gabriela Sandra; Montanaro, Mauro Aldo; et al.; Role of plasma membrane lipid composition on cellular homeostasis: learning from cell line models expressing fatty acid desaturases; Oxford University Press; Acta Biochimica Et Biophysica Sinica; 46; 4; 1-2014; 273-282
1005-9628
Autor
Jaureguiberry, María Soledad
Tricerri, Maria Alejandra
Sanchez, Susana A.
Finarelli, Gabriela Sandra
Montanaro, Mauro Aldo
Prieto, Eduardo Daniel
Rimoldi, Omar Jorge
Resumen
Experimental evidence has suggested that plasma membrane (PM)-associated signaling and hence cell metabolism and viability depend on lipid composition and organization. The aim of the present work is to develop a cell model to study the endogenous polyunsaturated fatty acids (PUFAs) effect on PM properties and analyze its influence on cholesterol (Chol) homeostasis. We have previously shown that by using a cell line over-expressing stearoyl-CoA-desaturase, membrane composition and organization coordinate cellular pathways involved in Chol efflux and cell viability by different mechanisms. Now, we expanded our studies to a cell model over-expressing both Δ5 and Δ6 desaturases, which resulted in a permanently higher PUFA content in PM. Furthermore, this cell line showed increased PM fluidity, Chol storage, and mitochondrial activity. In addition, human apolipoprotein A-I-mediated Chol removal was less efficient in these cells than in the corresponding control. Taken together, our results suggested that the cell functionality is preserved by regulating PM organization and Chol exportation and homeostasis.