Artículos de revistas
Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus
Fecha
2015-10Registro en:
Palazzolo, Martín Alejandro; Mascotti, María Laura; Lewkowicz, Elizabeth Sandra; Kurina Sanz, Marcela Beatriz; Self-sufficient redox biotransformation of lignin-related benzoic acids with Aspergillus flavus; Springer; Journal of Industrial Microbiology & Biotechnology; 42; 12; 10-2015; 1581-1589
1367-5435
Autor
Palazzolo, Martín Alejandro
Mascotti, María Laura
Lewkowicz, Elizabeth Sandra
Kurina Sanz, Marcela Beatriz
Resumen
Aromatic carboxylic acids are readily obtained from lignin in biomass processing facilities. However, efficient technologies for lignin valorization are missing. In this work, a microbial screening was conducted to find versatile biocatalysts capable of transforming several benzoic acids structurally related to lignin, employing vanillic acid as model substrate. The wild-type Aspergillus flavus growing cells exhibited exquisite selectivity towards the oxidative decarboxylation product, 2-methoxybenzene-1,4-diol. Interestingly, when assaying a set of structurally-related substrates, the biocatalyst displayed the oxidative removal of the carboxyl moiety or its reduction to the primary alcohol whether electron withdrawing or donating groups were present in the aromatic ring, respectively. Additionally, A. flavus proved to be highly tolerant to vanillic acid increasing concentrations (up to 8 g/L), demonstrating its potential application in chemical synthesis. A. flavus growing cells were found to be efficient biotechnological tools to perform self-sufficient, structure-dependent redox reactions. All the biotransformations described herein are reported for the first time.