dc.creator | Bertucci, Paola Yanina | |
dc.creator | Nacht, Ana Silvina | |
dc.creator | Alló, Mariano | |
dc.creator | Rocha Viegas, Luciana | |
dc.creator | Ballaré, Cecilia | |
dc.creator | Soronellas, Daniel | |
dc.creator | Castellano, Giancarlo | |
dc.creator | Zaurin, Roser | |
dc.creator | Kornblihtt, Alberto Rodolfo | |
dc.creator | Beato, Miguel | |
dc.creator | Vicent, Guillermo | |
dc.creator | Pecci, Adali | |
dc.date.accessioned | 2015-11-12T15:18:29Z | |
dc.date.accessioned | 2018-11-06T16:05:56Z | |
dc.date.available | 2015-11-12T15:18:29Z | |
dc.date.available | 2018-11-06T16:05:56Z | |
dc.date.created | 2015-11-12T15:18:29Z | |
dc.date.issued | 2013-05-02 | |
dc.identifier | Bertucci, Paola Yanina; Nacht, Ana Silvina; Alló, Mariano; Rocha Viegas, Luciana; Ballaré, Cecilia; et al.; Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation; Oxford University Press; Nucleic Acids Research; 41; 12; 2-5-2013; 6072-6086 | |
dc.identifier | 0305-1048 | |
dc.identifier | http://hdl.handle.net/11336/2748 | |
dc.identifier | 1362-4962 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1904579 | |
dc.description.abstract | Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of pro- gestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions sur- rounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-dis- tribution of the active Pol II toward the 30-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene ex- pression by facilitating the proper passage of the polymerase along hormone-dependent genes. | |
dc.language | eng | |
dc.publisher | Oxford University Press | |
dc.relation | info:eu-repo/semantics/altIdentifier/issn/0305-1048 | |
dc.relation | info:eu-repo/semantics/altIdentifier/issn/1362-4962 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://nar.oxfordjournals.org/content/41/12/6072.long | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695497/ | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093%2Fnar%2Fgkt327 | |
dc.rights | https://creativecommons.org/licenses/by-nc/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights | Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR) | |
dc.subject | PROGESTERONE | |
dc.subject | BCL-X | |
dc.subject | POLYMERASE 2 | |
dc.subject | ELONGATION | |
dc.title | Progesterone Receptor induces bcl-x expression through intragenic binding sites favoring RNA Polymerase II elongation | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |