Argentina
| Artículos de revistas
Simultaneous detection of bee viruses by multiplex PCR
dc.creator | Sguazza, Guillermo Hernán | |
dc.creator | Reynaldi, Francisco José | |
dc.creator | Galosi, Cecilia Monica | |
dc.creator | Pecoraro, Marcelo Ricardo | |
dc.date.accessioned | 2017-08-28T22:40:18Z | |
dc.date.accessioned | 2018-11-06T16:03:40Z | |
dc.date.available | 2017-08-28T22:40:18Z | |
dc.date.available | 2018-11-06T16:03:40Z | |
dc.date.created | 2017-08-28T22:40:18Z | |
dc.date.issued | 2013-08 | |
dc.identifier | Sguazza, Guillermo Hernán; Reynaldi, Francisco José; Galosi, Cecilia Monica; Pecoraro, Marcelo Ricardo; Simultaneous detection of bee viruses by multiplex PCR ; Elsevier Science; Journal of Virological Methods; 194; 1-2; 8-2013; 102-106 | |
dc.identifier | 0166-0934 | |
dc.identifier | http://hdl.handle.net/11336/23204 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1904115 | |
dc.description.abstract | Honey bee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. Itis known thatthe consequence ofthe complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are detected frequently concomitantly in bee colonies. The aim of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of the most prevalent bee viruses. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of a particular virus as well as multiple virus infections in a single reaction tube. This method could be a helpful tool in the surveillance of the most frequently found bee viruses and to study the dynamics and the interactions of the virus populations within colonies. | |
dc.language | eng | |
dc.publisher | Elsevier Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093413003352 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jviromet.2013.08.003 | |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | Apis mellifera | |
dc.subject | Bee viruses | |
dc.subject | Multiplex RT-PCR | |
dc.subject | Detection | |
dc.title | Simultaneous detection of bee viruses by multiplex PCR | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas |