Argentina | Artículos de revistas
dc.creatorSguazza, Guillermo Hernán
dc.creatorReynaldi, Francisco José
dc.creatorGalosi, Cecilia Monica
dc.creatorPecoraro, Marcelo Ricardo
dc.date.accessioned2017-08-28T22:40:18Z
dc.date.accessioned2018-11-06T16:03:40Z
dc.date.available2017-08-28T22:40:18Z
dc.date.available2018-11-06T16:03:40Z
dc.date.created2017-08-28T22:40:18Z
dc.date.issued2013-08
dc.identifierSguazza, Guillermo Hernán; Reynaldi, Francisco José; Galosi, Cecilia Monica; Pecoraro, Marcelo Ricardo; Simultaneous detection of bee viruses by multiplex PCR ; Elsevier Science; Journal of Virological Methods; 194; 1-2; 8-2013; 102-106
dc.identifier0166-0934
dc.identifierhttp://hdl.handle.net/11336/23204
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1904115
dc.description.abstractHoney bee mortality is a serious problem that beekeepers in Argentina have had to face during the last 3 years. Itis known thatthe consequence ofthe complex interactions between environmental and beekeeping parameters added to the effect of different disease agents such as viruses, bacteria, fungi and parasitic mites may result in a sudden collapse of the colony. In addition, multiple viral infections are detected frequently concomitantly in bee colonies. The aim of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of the most prevalent bee viruses. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of a particular virus as well as multiple virus infections in a single reaction tube. This method could be a helpful tool in the surveillance of the most frequently found bee viruses and to study the dynamics and the interactions of the virus populations within colonies.
dc.languageeng
dc.publisherElsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166093413003352
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jviromet.2013.08.003
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectApis mellifera
dc.subjectBee viruses
dc.subjectMultiplex RT-PCR
dc.subjectDetection
dc.titleSimultaneous detection of bee viruses by multiplex PCR
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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