dc.creator | Recouvreux, Maria Victoria | |
dc.creator | Lapyckyj, Lara | |
dc.creator | Camilletti, María Andrea | |
dc.creator | Guida, María Clara | |
dc.creator | Ornstein, Ana Maria | |
dc.creator | Rifkin, Daniel B. | |
dc.creator | Becu, Damasia | |
dc.creator | Diaz, Graciela Susana | |
dc.date.accessioned | 2015-09-30T16:00:22Z | |
dc.date.available | 2015-09-30T16:00:22Z | |
dc.date.created | 2015-09-30T16:00:22Z | |
dc.date.issued | 2013-09-05 | |
dc.identifier | Recouvreux, Maria Victoria; Lapyckyj, Lara; Camilletti, María Andrea; Guida, María Clara; Ornstein, Ana Maria; et al.; Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas; Endocrine Soc; Endocrinology; 154; 11; 5-9-2013; 4192-4205 | |
dc.identifier | 0013-7227 | |
dc.identifier | http://hdl.handle.net/11336/2212 | |
dc.identifier | 1945-7170 | |
dc.description.abstract | Dopamine and estradiol interact in the regulation of lactotroph cell proliferation and prolactin secretion. Ablation of the dopamine D2 receptor gene (Drd2 / ) in mice leads to a sexually dimorphic phenotype of hyperprolactinemia and pituitary hyperplasia, which is stronger in females. TGF- 1 is a known inhibitor of lactotroph proliferation. TGF- 1 is regulated by dopamine and estradiol, and it is usually down-regulated in prolactinoma experimental models. To understand the role of TGF- 1 in the gender-specific development of prolactinomas in Drd2 / mice, we compared the expression of different components of the pituitary TGF- 1 system, including active cytokine content, latent TGF- –binding protein isoforms, and possible local TGF- 1 activators, in males and females in this model. Furthermore, we evaluated the effects of dopamine and estradiol administration to elucidate their role in TGF- 1 system regulation. The expression of active TGF- 1, latent TGF- –binding protein isoforms, and several putative TGF- 1 activators evaluated was higher in male than in female mouse pituitary glands. However, Drd2 / female mice were more sensitive to the decrease in active TGF- 1 content, as reflected by the down-regulation of TGF- 1 target genes. Estrogen and dopamine caused differential regulation of several components of the TGF- 1 system. In particular, we found sex- and genotype- dependent regulation of active TGF- 1 content and a similar expression pattern for 2 of the putative TGF- 1 activators, thrombospondin-1 and kallikrein-1, suggesting that these proteins could mediate TGF- 1 activation elicited by dopamine and estradiol. Our results indicate that (1) the loss of dopaminergic tone affects the pituitary TGF- 1 system more strongly in females than in males, (2) males express higher levels of pituitary TGF- 1 system components including active cytokine, and (3) estradiol negatively controls most of the components of the system. Because TGF- 1 inhibits lactotroph proliferation, we propose that the higher levels of the TGF- 1 system in males could protect or delay the development of prolactinomas in Drd2 / male mice. | |
dc.language | eng | |
dc.publisher | Endocrine Soc | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800752/ | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://press.endocrine.org/doi/pdf/10.1210/en.2013-1433 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Prolactin | |
dc.subject | Tgf Beta | |
dc.subject | Pituitary | |
dc.subject | Sex Differences | |
dc.title | Sex differences in the pituitary transforming growth factor-beta1 (TGF-beta1) system: studies in a model of resistant prolactinomas | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |