dc.creatorPanei, Carlos Javier
dc.creatorTakeshima, Shin-nosuke
dc.creatorOmori, Takashi
dc.creatorNunoya, Tetsuo
dc.creatorDavis, William C.
dc.creatorIshizaki, Hiroshi
dc.creatorMatoba, Kazuhiro
dc.creatorAida, Yoko
dc.date.accessioned2018-04-10T20:24:42Z
dc.date.accessioned2018-11-06T16:01:26Z
dc.date.available2018-04-10T20:24:42Z
dc.date.available2018-11-06T16:01:26Z
dc.date.created2018-04-10T20:24:42Z
dc.date.issued2013-03
dc.identifierPanei, Carlos Javier; Takeshima, Shin-nosuke; Omori, Takashi; Nunoya, Tetsuo; Davis, William C.; et al.; Estimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR; BioMed Central; BMC Veterinary Research; 9; 95; 3-2013; 1-8
dc.identifier1746-6148
dc.identifierhttp://hdl.handle.net/11336/41613
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1903636
dc.description.abstractBovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most commonneoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistentlymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+Tcells,CD3+Tcells,CD4+Tcells,CD8+Tcells,γ/δT cells, monocytes, and granulocytes in infected cattle that do not have tumors,although the most consistently infected cell is the CD5+B cell. The mechanism by which BLV causes uncontrolledCD5+B cell proliferation is unknown. Recently, we developed a new quantitative real-time polymerase chainreaction (PCR) method, BLV-CoCoMo-qPCR, which enabled us to demonstrate that the proviral load correlates notonly with BLV infection, as assessed by syncytium formation, but also with BLV disease progression. The presentstudy reports the distribution of BLV provirus in peripheral blood mononuclear cell subpopulations isolated fromBLV-infected cows at the subclinical stage of EBL as examined by cell sorting and BLV-CoCoMo-qPCR
dc.languageeng
dc.publisherBioMed Central
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-9-95
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1746-6148-9-95
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBovine leukemia virus (BLV)
dc.subjectproviral load
dc.subjectBLV-CoCoMo-qPCR
dc.subjectCD5+IgM+ cell
dc.subjectflow cytometry
dc.subjectcell sorting
dc.titleEstimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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