Artículos de revistas
Understanding the function of bacterial and eukaryotic thiolases II by integrating evolutionary and functional approaches
Fecha
2013-10-11Registro en:
Fox, Ana Romina; Soto, Gabriela Cynthia; Mozzicafreddo, Matteo; García, Araceli Nora; Cuccioloni, Massimiliano; et al.; Understanding the function of bacterial and eukaryotic thiolases II by integrating evolutionary and functional approaches; Elsevier; Gene; 533; 1; 11-10-2013; 5-10
0378-1119
Autor
Fox, Ana Romina
Soto, Gabriela Cynthia
Mozzicafreddo, Matteo
García, Araceli Nora
Cuccioloni, Massimiliano
Angeletti, Mauro
Salerno, Juan Carlos
Ayub, Nicolas Daniel
Resumen
Acetoacetyl-CoA thiolase (EC 2.3.1.9), commonly named thiolase II, condenses two molecules of acetyl-CoA to give acetoacetyl-CoA and CoA. This enzyme acts in anabolic processes as the first step in the biosynthesis of isoprenoids and polyhydroxybutyrate in eukaryotes and bacteria, respectively. We have recently reported the evolutionary and functional equivalence of these enzymes, suggesting that thiolase II could be the rate limiting enzyme in these pathways and presented evidence indicating that this enzyme modulates the availability of reducing equivalents during abiotic stress adaptation in bacteria and plants. However, these results are not sufficient to clarify why thiolase II was evolutionary selected as a critical enzyme in the production of antioxidant compounds. Regarding this intriguing topic, we propose that thiolase II could sense changes in the acetyl-CoA/CoA ratio induced by the inhibition of the tricarboxylic acid cycle under abiotic stress. Thus, the high level of evolutionary and functional constraint of thiolase II may be due to the connection of this enzyme with an ancient and conserved metabolic route.