dc.creatorBertucci, Juan Ignacio
dc.creatorBlanco, Ayelén Melisa
dc.creatorCanosa, Luis Fabian
dc.creatorUnniappan, Suraj
dc.date.accessioned2018-06-22T20:42:41Z
dc.date.accessioned2018-11-06T15:43:42Z
dc.date.available2018-06-22T20:42:41Z
dc.date.available2018-11-06T15:43:42Z
dc.date.created2018-06-22T20:42:41Z
dc.date.issued2017-09
dc.identifierBertucci, Juan Ignacio; Blanco, Ayelén Melisa; Canosa, Luis Fabian; Unniappan, Suraj; Direct actions of macronutrient components on goldfish hepatopancreas in vitro to modulate the expression of ghr-I, ghr-II, igf-I and igf-II mRNAs; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 250; 9-2017; 1-8
dc.identifier0016-6480
dc.identifierhttp://hdl.handle.net/11336/49796
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1900279
dc.description.abstractIn mammals and fish, somatic growth and metabolism are coordinated by the GH-IGF axis, composed of growth hormone (GH), growth hormone receptors I and II (GHR-I and GHR-II), and the insulin-like growth factors I and II (IGF-I and IGF-II). In order to determine if dietary macronutrients regulate the hepatopancreatic expression of ghr-I, ghr-II, igf-I and igf-II independently of circulating GH, organ culture experiments were conducted. Briefly, goldfish hepatopancreas sections were incubated with different doses of glucose; L-tryptophan; oleic acid; linolenic acid (LNA); eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). After two and four hours of treatment, the expression of ghr-I, ghr-II, igf-I and igf-II mRNAs was quantified. We found that glucose and L-tryptophan globally upregulate the mRNA expression of ghr-I; ghr-II; igf-I and igf-II. Duration of exposure, and unsaturation level of fatty acids differentially modulate ghr-I, ghr-II and igf-II mRNA expression. Additionally, we found that fatty acids increase the expression of igf-I depending on incubation time and fatty acid class. In conclusion, here we present evidence for GH-independent, direct effects exerted by dietary macronutrients on GHR and IGF in goldfish hepatopancreas.
dc.languageeng
dc.publisherAcademic Press Inc Elsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ygcen.2017.05.014
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0016648017301338
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectFATTY ACID
dc.subjectGLUCOSE
dc.subjectGROWTH HORMONE RECEPTOR
dc.subjectINSLULINE-LIKE GROWTH FACTOR
dc.subjectMETABOLISM
dc.subjectTRYPTOPHAN
dc.titleDirect actions of macronutrient components on goldfish hepatopancreas in vitro to modulate the expression of ghr-I, ghr-II, igf-I and igf-II mRNAs
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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