dc.creator | Bertucci, Juan Ignacio | |
dc.creator | Blanco, Ayelén Melisa | |
dc.creator | Canosa, Luis Fabian | |
dc.creator | Unniappan, Suraj | |
dc.date.accessioned | 2018-06-22T20:42:41Z | |
dc.date.accessioned | 2018-11-06T15:43:42Z | |
dc.date.available | 2018-06-22T20:42:41Z | |
dc.date.available | 2018-11-06T15:43:42Z | |
dc.date.created | 2018-06-22T20:42:41Z | |
dc.date.issued | 2017-09 | |
dc.identifier | Bertucci, Juan Ignacio; Blanco, Ayelén Melisa; Canosa, Luis Fabian; Unniappan, Suraj; Direct actions of macronutrient components on goldfish hepatopancreas in vitro to modulate the expression of ghr-I, ghr-II, igf-I and igf-II mRNAs; Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 250; 9-2017; 1-8 | |
dc.identifier | 0016-6480 | |
dc.identifier | http://hdl.handle.net/11336/49796 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1900279 | |
dc.description.abstract | In mammals and fish, somatic growth and metabolism are coordinated by the GH-IGF axis, composed of growth hormone (GH), growth hormone receptors I and II (GHR-I and GHR-II), and the insulin-like growth factors I and II (IGF-I and IGF-II). In order to determine if dietary macronutrients regulate the hepatopancreatic expression of ghr-I, ghr-II, igf-I and igf-II independently of circulating GH, organ culture experiments were conducted. Briefly, goldfish hepatopancreas sections were incubated with different doses of glucose; L-tryptophan; oleic acid; linolenic acid (LNA); eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). After two and four hours of treatment, the expression of ghr-I, ghr-II, igf-I and igf-II mRNAs was quantified. We found that glucose and L-tryptophan globally upregulate the mRNA expression of ghr-I; ghr-II; igf-I and igf-II. Duration of exposure, and unsaturation level of fatty acids differentially modulate ghr-I, ghr-II and igf-II mRNA expression. Additionally, we found that fatty acids increase the expression of igf-I depending on incubation time and fatty acid class. In conclusion, here we present evidence for GH-independent, direct effects exerted by dietary macronutrients on GHR and IGF in goldfish hepatopancreas. | |
dc.language | eng | |
dc.publisher | Academic Press Inc Elsevier Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ygcen.2017.05.014 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0016648017301338 | |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | FATTY ACID | |
dc.subject | GLUCOSE | |
dc.subject | GROWTH HORMONE RECEPTOR | |
dc.subject | INSLULINE-LIKE GROWTH FACTOR | |
dc.subject | METABOLISM | |
dc.subject | TRYPTOPHAN | |
dc.title | Direct actions of macronutrient components on goldfish hepatopancreas in vitro to modulate the expression of ghr-I, ghr-II, igf-I and igf-II mRNAs | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |