dc.creator | Dolce, Natalia Raquel | |
dc.creator | Mroginski, Luis Amado | |
dc.creator | Rey, Hebe Yolanda | |
dc.date.accessioned | 2015-11-17T18:11:33Z | |
dc.date.accessioned | 2018-11-06T15:41:59Z | |
dc.date.available | 2015-11-17T18:11:33Z | |
dc.date.available | 2018-11-06T15:41:59Z | |
dc.date.created | 2015-11-17T18:11:33Z | |
dc.date.issued | 2011-02 | |
dc.identifier | Dolce, Natalia Raquel; Mroginski, Luis Amado; Rey, Hebe Yolanda; Enhanced seed germination of Ilex dumosa R. (Aquifoliaceae) through in vitro culture of cut pyrenes; Amer Soc Horticultural Science; Hortscience; 46; 2; 2-2011; 278–281 | |
dc.identifier | 0018-5345 | |
dc.identifier | http://hdl.handle.net/11336/2848 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1899909 | |
dc.description.abstract | An in vitro culture protocol was developed that increased the germination percentage and decreased the lag time to germination for Ilex dumosa R. pyrenes as a tool for replacing the laborious task of embryo rescue technique. This method involves transversely cutting surface-sterilized pyrenes with a scalpel blade, then placing the micropylar one-third end with the rudimentary embryo (0.25 mm long) on solidified (agar 0.65%) quarter-strength salts and vitamins of Murashige and Skoog, 1962 medium with 3% sucrose, and incubating in a growth room at 27 ± 2 8C with a 14-h photoperiod (116 mmolm?2 s?1). Most of the cut pyrenes (greater than 50%) germinated within the first month after inoculation and achieved maximum germination (70%) in 2 months compared with whole pyrenes, which began to germinate 3 months after sowing and required more than 8 months for maximum germination (37%). Moreover, the germination percentage of cut pyrenes was significantly higher than the germination of isolated embryos (34%). Thus, the cut pyrenes culture is a simpler and more effective technique than embryo rescue. Easily, on average, a trained operator is able to culture ~1000 cut pyrenes per day instead of ~100 isolated embryos. | |
dc.language | eng | |
dc.publisher | Amer Soc Horticultural Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://hortsci.ashspublications.org/content/46/2/278.full.pdf+html | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights | Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR) | |
dc.subject | EMBRYO RESCUE | |
dc.subject | SEED DORMANCY | |
dc.subject | LIGHT | |
dc.subject | COLD STORAGE | |
dc.subject | HOLLY | |
dc.title | Enhanced seed germination of Ilex dumosa R. (Aquifoliaceae) through in vitro culture of cut pyrenes | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |