dc.creatorDolce, Natalia Raquel
dc.creatorMroginski, Luis Amado
dc.creatorRey, Hebe Yolanda
dc.date.accessioned2015-11-17T18:11:33Z
dc.date.accessioned2018-11-06T15:41:59Z
dc.date.available2015-11-17T18:11:33Z
dc.date.available2018-11-06T15:41:59Z
dc.date.created2015-11-17T18:11:33Z
dc.date.issued2011-02
dc.identifierDolce, Natalia Raquel; Mroginski, Luis Amado; Rey, Hebe Yolanda; Enhanced seed germination of Ilex dumosa R. (Aquifoliaceae) through in vitro culture of cut pyrenes; Amer Soc Horticultural Science; Hortscience; 46; 2; 2-2011; 278–281
dc.identifier0018-5345
dc.identifierhttp://hdl.handle.net/11336/2848
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1899909
dc.description.abstractAn in vitro culture protocol was developed that increased the germination percentage and decreased the lag time to germination for Ilex dumosa R. pyrenes as a tool for replacing the laborious task of embryo rescue technique. This method involves transversely cutting surface-sterilized pyrenes with a scalpel blade, then placing the micropylar one-third end with the rudimentary embryo (0.25 mm long) on solidified (agar 0.65%) quarter-strength salts and vitamins of Murashige and Skoog, 1962 medium with 3% sucrose, and incubating in a growth room at 27 ± 2 8C with a 14-h photoperiod (116 mmolm?2 s?1). Most of the cut pyrenes (greater than 50%) germinated within the first month after inoculation and achieved maximum germination (70%) in 2 months compared with whole pyrenes, which began to germinate 3 months after sowing and required more than 8 months for maximum germination (37%). Moreover, the germination percentage of cut pyrenes was significantly higher than the germination of isolated embryos (34%). Thus, the cut pyrenes culture is a simpler and more effective technique than embryo rescue. Easily, on average, a trained operator is able to culture ~1000 cut pyrenes per day instead of ~100 isolated embryos.
dc.languageeng
dc.publisherAmer Soc Horticultural Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://hortsci.ashspublications.org/content/46/2/278.full.pdf+html
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.subjectEMBRYO RESCUE
dc.subjectSEED DORMANCY
dc.subjectLIGHT
dc.subjectCOLD STORAGE
dc.subjectHOLLY
dc.titleEnhanced seed germination of Ilex dumosa R. (Aquifoliaceae) through in vitro culture of cut pyrenes
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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