Artículos de revistas
An in vitro and in vivo evaluation of new potential trans-sialidase inhibitors of Trypanosoma cruzi predicted by a computational drug repositioning method
Fecha
2017-05Registro en:
Lara Ramirez, Edgar E.; López Cedillo, Julio Cesar; Nogueda Torres, Benjamin; Kashif, Muhammad; Garcia Perez, Carlos; et al.; An in vitro and in vivo evaluation of new potential trans-sialidase inhibitors of Trypanosoma cruzi predicted by a computational drug repositioning method; Elsevier France-editions Scientifiques Medicales Elsevier; European Journal of Medical Chemistry; 132; 5-2017; 249-261
0223-5234
CONICET Digital
CONICET
Autor
Lara Ramirez, Edgar E.
López Cedillo, Julio Cesar
Nogueda Torres, Benjamin
Kashif, Muhammad
Garcia Perez, Carlos
Bocanegra Garcia, Virgilio
Agusti, Rosalia
Uhrig, Maria Laura
Rivera, Gildardo
Resumen
Chagas disease is one of the most important neglected parasitic diseases afflicting developed and undeveloped countries. There are currently limited options for inexpensive and secure pharmacological treatment. In this study, we employed a structure-based virtual screening protocol for 3180 FDA-approved drugs for repositioning of them as potential trans-sialidase inhibitors. In vitro and in vivo evaluations were performed for the selected drugs against trypomastigotes from the INC-5 and NINOA strains of T. cruzi. Also, inhibition of sialylation by the trans-sialidase enzyme reaction was evaluated using high-performance anion-exchange chromatography with pulse amperometric detection to confirm the mechanism of action. Results from the computational study showed 38 top drugs with the best binding-energies. Four compounds with antihistaminic, anti-hypertensive, and antibiotic properties showed better trypanocidal effects (LC50 range = 4.5–25.8 μg/mL) than the reference drugs, nifurtimox and benznidazole (LC50 range = 36.1–46.8 μg/mL) in both strains in the in vitro model. The anti-inflammatory, sulfasalazine showed moderate inhibition (37.6%) of sialylation in a trans-sialidase enzyme inhibition reaction. Sulfasalazine also showed the best trypanocidal effects in short-term in vivo experiments on infected mice. This study suggests for the first time that the anti-inflammatory sulfasalazine could be used as a lead compound to develop new trans-sialidase inhibitors.