dc.creator | Barberis, Claudia | |
dc.creator | Almuzara, Marisa | |
dc.creator | Joint Lambert, Olivier | |
dc.creator | Ramirez, Maria Soledad | |
dc.creator | Famiglietti, Angela María Rosa | |
dc.creator | Vay, Carlos Alberto | |
dc.date.accessioned | 2017-12-14T18:47:25Z | |
dc.date.available | 2017-12-14T18:47:25Z | |
dc.date.created | 2017-12-14T18:47:25Z | |
dc.date.issued | 2014-09 | |
dc.identifier | Barberis, Claudia; Almuzara, Marisa; Joint Lambert, Olivier; Ramirez, Maria Soledad; Famiglietti, Angela María Rosa; et al.; Comparison of the Bruker MALDI-TOF Mass Spectrometry System and Conventional Phenotypic Methods for Identification of Gram-Positive Rods; Public Library of Science; Plos One; 9; 9; 9-2014; 1-6; e106303-e106309 | |
dc.identifier | 1932-6203 | |
dc.identifier | http://hdl.handle.net/11336/30685 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.description.abstract | In recent years, MALDI-TOF Mass Spectrometry (MS) method has emerged as a promising and a reliable tool for bacteria identification. In this study we compared Bruker MALDI-TOF MS and conventional phenotypic methods to identify a collection of 333 Gram-positive clinical isolates comprising 22 genera and 60 species. 16S rRNA sequencing was the reference molecular technique, and rpoB gene sequecing was used as a secondary gene target when 16Sr RNA did not allow species identification of Corynebacterium spp. We also investigate if score cut-offs values of ≥1,5 and ≥1,7 were accurate for genus and species-level identification using the Bruker system. Identification at species level was obtained for 92,49% of Gram-positive rods by MALDI-TOF MS compared to 85,89% by phenotypic method. Our data validates the score ≥1,5 for genus level and ≥1,7 for species-level identification in a large and diverse collection of Gram-positive rods. The present study has proved the accuracy of MALDI-TOF MS as an identification method in Gram-positive rods compared to currently used methods in routine laboratories. | |
dc.language | eng | |
dc.publisher | Public Library of Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0106303 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0106303 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153636/ | |
dc.rights | https://creativecommons.org/licenses/by/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Maldi-Tof Ms | |
dc.subject | Corynebacterium Sp | |
dc.title | Comparison of the Bruker MALDI-TOF Mass Spectrometry System and Conventional Phenotypic Methods for Identification of Gram-Positive Rods | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |