dc.creatorBarberis, Claudia
dc.creatorAlmuzara, Marisa
dc.creatorJoint Lambert, Olivier
dc.creatorRamirez, Maria Soledad
dc.creatorFamiglietti, Angela María Rosa
dc.creatorVay, Carlos Alberto
dc.date.accessioned2017-12-14T18:47:25Z
dc.date.available2017-12-14T18:47:25Z
dc.date.created2017-12-14T18:47:25Z
dc.date.issued2014-09
dc.identifierBarberis, Claudia; Almuzara, Marisa; Joint Lambert, Olivier; Ramirez, Maria Soledad; Famiglietti, Angela María Rosa; et al.; Comparison of the Bruker MALDI-TOF Mass Spectrometry System and Conventional Phenotypic Methods for Identification of Gram-Positive Rods; Public Library of Science; Plos One; 9; 9; 9-2014; 1-6; e106303-e106309
dc.identifier1932-6203
dc.identifierhttp://hdl.handle.net/11336/30685
dc.identifierCONICET Digital
dc.identifierCONICET
dc.description.abstractIn recent years, MALDI-TOF Mass Spectrometry (MS) method has emerged as a promising and a reliable tool for bacteria identification. In this study we compared Bruker MALDI-TOF MS and conventional phenotypic methods to identify a collection of 333 Gram-positive clinical isolates comprising 22 genera and 60 species. 16S rRNA sequencing was the reference molecular technique, and rpoB gene sequecing was used as a secondary gene target when 16Sr RNA did not allow species identification of Corynebacterium spp. We also investigate if score cut-offs values of ≥1,5 and ≥1,7 were accurate for genus and species-level identification using the Bruker system. Identification at species level was obtained for 92,49% of Gram-positive rods by MALDI-TOF MS compared to 85,89% by phenotypic method. Our data validates the score ≥1,5 for genus level and ≥1,7 for species-level identification in a large and diverse collection of Gram-positive rods. The present study has proved the accuracy of MALDI-TOF MS as an identification method in Gram-positive rods compared to currently used methods in routine laboratories.
dc.languageeng
dc.publisherPublic Library of Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0106303
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0106303
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153636/
dc.rightshttps://creativecommons.org/licenses/by/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMaldi-Tof Ms
dc.subjectCorynebacterium Sp
dc.titleComparison of the Bruker MALDI-TOF Mass Spectrometry System and Conventional Phenotypic Methods for Identification of Gram-Positive Rods
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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