Artículos de revistas
Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan
Fecha
2017-03Registro en:
Campos, Débora A.; Woitovich Valetti, Nadia; Oliveira, Ana; Pastrana Castro, Lorenzo M.; Teixeira, José A.; et al.; Platform design for extraction and isolation of Bromelain: Complex formation and precipitation with carrageenan; Elsevier; Process Biochemistry; 54; 3-2017; 156-161
1359-5113
CONICET Digital
CONICET
Autor
Campos, Débora A.
Woitovich Valetti, Nadia
Oliveira, Ana
Pastrana Castro, Lorenzo M.
Teixeira, José A.
Pintado, Maria Manuela
Picó, Guillermo Alfredo
Resumen
The main objective of this work was to investigate for the first time the molecular mechanism of complex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strong polyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. The Bromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility was dependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH was changed and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism for the formation of non-soluble complex. The formation of the carrageenan-bromelain complex increased in 8 °C the enzyme thermal stability, while its biological activity was not modified. The amount of total enzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 85–90%.