Artículos de revistas
The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation
Fecha
2004-12Registro en:
Shayo, Carina Claudia; Lemos Legnazzi, Bibiana; Monczor, Federico; Fernández, Natalia Brenda; Riveiro, María Eugenia; et al.; The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation; Elsevier Inc; Biochemical and Biophysical Research Communications; 314; 3; 12-2004; 798-804
0006-291X
1090-2104
CONICET Digital
CONICET
Autor
Shayo, Carina Claudia
Lemos Legnazzi, Bibiana
Monczor, Federico
Fernández, Natalia Brenda
Riveiro, María Eugenia
Baldi, Alberto
Davio, Carlos Alberto
Resumen
The regulation of the cAMP signaling is intimately involved in several cellular processes, including cell differentiation. Here, we provide strong evidence supporting that the time-course of cAMP signal is critical for leukemia U-937 cell differentiation. Three stimulating-cAMP agents were used to analyze the correlation between cAMP time-course and cell differentiation. All three agents denoted similar cAMP maximal responses in dose-response experiments. The kinetic of desensitization showed differential characteristics, while H2 receptor desensitized homologously without affecting PGE2 or forskolin effect, PGE2 response showed mixed desensitization characterized by a homologous initial phase followed by a heterologous phase. Regarding forskolin, long-term stimuli attenuated PGE2 and H2 agonist response without affecting adenylyl cyclase activity. In the absence of phosphodiesterase inhibitors, the three agents induced similar maximal cAMP levels after 5 min, but only that induced by the H2 agonist returned to basal levels. Consistent with this observation, H2 agonist was not able to induce U-937 cell maturation in contrast to PGE2 and forskolin, supporting the importance of time-course signaling in the determination of cell behavior.