dc.creator | Ricatti, Maria Jimena | |
dc.creator | Battista, Ariadna Gabriela | |
dc.creator | Zorrilla Zubilete, María Aurelia | |
dc.creator | Faillace, Maria Paula | |
dc.date.accessioned | 2017-06-14T21:31:45Z | |
dc.date.available | 2017-06-14T21:31:45Z | |
dc.date.created | 2017-06-14T21:31:45Z | |
dc.date.issued | 2011-04 | |
dc.identifier | Ricatti, Maria Jimena; Battista, Ariadna Gabriela; Zorrilla Zubilete, María Aurelia; Faillace, Maria Paula; Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina; SAGE Publications; Journal of Biological Rhythms; 26; 2; 4-2011; 107-117 | |
dc.identifier | 0748-7304 | |
dc.identifier | http://hdl.handle.net/11336/18222 | |
dc.identifier | 1552-4531 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.description.abstract | Regeneration and growth that occur in the adult teleost retina have been helpful in identifying molecular and cellular mechanisms underlying cell proliferation and differentiation. Here, it is reported that S-phase cell number, in the ciliary marginal zone (CMZ) of the adult zebrafish retina, exhibits day-night variations with a mid-light phase peak. Oscillations persist for 24 h in constant darkness (DD), suggesting control by a circadian component. However, variations in the S-phase nuclei number were rapidly dampened and not present during and after a second day in DD. An ADPβS treatment significantly enhanced S-phase activity at night to mid-light levels, as assessed by in vivo BrdU incorporation in a 2-h interval. Moreover, daylight increase in S-phase cell number was completely abolished when extracellular nucleotide levels or their extracellular hydrolysis by ectonucleoside triphosphate diphosphohydrolases (NTPDases) were significantly disrupted or when a selective antagonist of purinergic P2Y1 receptors was intraocularly injected before BrdU exposure. Extracellular nucleotides and NTPDase action were also important for maintaining nocturnal low levels of S-phase activity in the CMZ. Finally, we showed that mRNAs of NTPDases 1, 2 (3 isoforms), and 3 as well as of P2Y1 receptor are present in the neural retina of zebrafish. NTPDase mRNA expression exhibited a 2-fold increment in light versus dark conditions as assessed by quantitative RT-PCR, whereas P2Y1 receptor mRNA levels did not show significant day-night variations. This study demonstrates a key role for nucleotides, principally ADP as a paracrine signal, as well as for NTPDases, the plasma membrane—bound enzymes that control extracellular nucleotide concentration, for inducing S-phase cell entry in the CMZ—normally associated with retinal growth—throughout the light-dark cycle. | |
dc.language | eng | |
dc.publisher | SAGE Publications | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/0748730410395528 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/0748730410395528 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | CILIARY MARGINAL ZONE | |
dc.subject | EXTRACELLULAR NUCLEOTIDES | |
dc.subject | S-PHASE CELL RHYTHM | |
dc.subject | ZEBRAFISH RETINA | |
dc.title | Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |