info:eu-repo/semantics/article
Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development
Fecha
2016-07Registro en:
Coluccio Leskow, Carla; Kamenetzky, Laura; Dominguez, Pia Guadalupe; Diaz Zirpolo, Jose Antonio; Obata, Toshihiro; et al.; Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development; Oxford University Press; Journal of Experimental Botany; 67; 14; 7-2016; 4091-4103
0022-0957
1460-2431
CONICET Digital
CONICET
Autor
Coluccio Leskow, Carla
Kamenetzky, Laura
Dominguez, Pia Guadalupe
Diaz Zirpolo, Jose Antonio
Obata, Toshihiro
Costa, Hernán
Marti, Marcelo Adrian
Taboga, Oscar Alberto
Keurentjes, Joost
Sulpice, Ronan
Ishihara, Hirofumi
Stitt, Mark
Fernie, Alisdair Robert
Carrari, Fernando Oscar
Resumen
Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar Km for both alleles and a slightly higher Vmax for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vacInv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.