dc.creator | Muglia, Cecilia Isabel | |
dc.creator | Papa Gobbi, Rodrigo | |
dc.creator | Smaldini, Paola Lorena | |
dc.creator | Orsini Delgado, María Lucía | |
dc.creator | Candia, Martín | |
dc.creator | Zanuzzi, Carolina Natalia | |
dc.creator | Sambuelli, Alicia | |
dc.creator | Rocca, Andrés | |
dc.creator | Toscano, Marta Alicia | |
dc.creator | Rabinovich, Gabriel Adrián | |
dc.creator | Docena, Guillermo H. | |
dc.date.accessioned | 2018-08-14T22:01:25Z | |
dc.date.accessioned | 2018-11-06T15:04:59Z | |
dc.date.available | 2018-08-14T22:01:25Z | |
dc.date.available | 2018-11-06T15:04:59Z | |
dc.date.created | 2018-08-14T22:01:25Z | |
dc.date.issued | 2016-07 | |
dc.identifier | Muglia, Cecilia Isabel; Papa Gobbi, Rodrigo; Smaldini, Paola Lorena; Orsini Delgado, María Lucía; Candia, Martín; et al.; Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Physiology; 231; 7; 7-2016; 1575-1585 | |
dc.identifier | 0021-9541 | |
dc.identifier | http://hdl.handle.net/11336/55530 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1893630 | |
dc.description.abstract | Galectins play key roles in the inflammatory cascade. In this study, we aimed to analyze the effect of galectin-1 (Gal-1) in the function of intestinal epithelial cells (IECs) isolated from healthy and inflamed mucosa. IECs isolated from mice or patients with inflammatory bowel diseases (IBD) were incubated with different pro-inflammatory cytokines, and Gal-1 binding, secretion of homeostatic factors and viability were assessed. Experimental models of food allergy and colitis were used to evaluate the in vivo influence of inflammation on Gal-1 binding and modulation of IECs. We found an enhanced binding of Gal-1 to: (a) murine IECs exposed to IL-1β, TNF, and IL-13; (b) IECs from inflamed areas in intestinal tissue from IBD patients; (c) small bowel of allergic mice; and (d) colon from mice with experimental colitis. Our results showed that low concentrations of Gal-1 favored a tolerogenic micro-environment, whereas high concentrations of this lectin modulated viability of IECs through mechanisms involving activation of caspase-9 and modulation of Bcl-2 protein family members. Our results showed that, when added in the presence of diverse pro-inflammatory cytokines such as tumor necrosis factor (TNF), IL-13 and IL-5, Gal-1 differentially promoted the secretion of growth factors including thymic stromal lymphopoietin (TSLP), epidermal growth factor (EGF), IL-10, IL-25, and transforming growth factor (TGF-β1). In conclusion, we found an augmented binding of Gal-1 to IECs when exposed in vitro or in vivo to inflammatory stimuli, showing different effects depending on Gal-1 concentration. These findings highlight the importance of the inflammatory micro-environment of mucosal tissues in modulating IECs susceptibility to the immunoregulatory lectin Gal-1 and its role in epithelial cell homeostasis. | |
dc.language | eng | |
dc.publisher | Wiley-liss, Div John Wiley & Sons Inc | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jcp.25249 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.25249 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | ALLERGY | |
dc.subject | COLITIS | |
dc.subject | GALECTIN-1 | |
dc.subject | GUT | |
dc.subject | INFLAMMATION | |
dc.title | Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1 | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |