Artículos de revistas
Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain
Fecha
2012-11Registro en:
Konrath, Eduardo Luis; Neves, Bruna Medeiros; Passos, Carolina Dos Santos; Lunardi, Paula Santana; Ortega, María Gabriela; et al.; Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain; Elsevier Gmbh; Phytomedicine; 19; 14; 11-2012; 1321-1324
0944-7113
1618-095X
CONICET Digital
CONICET
Autor
Konrath, Eduardo Luis
Neves, Bruna Medeiros
Passos, Carolina Dos Santos
Lunardi, Paula Santana
Ortega, María Gabriela
Cabrera, Jose Luis
Goncalves, C. A.
Henriques, Amalia Teresinha
Resumen
Huperzine A, a Lycopodium alkaloid produced by Chinese folk herb Huperzia serrata (Lycopodiaceae), has been shown to be a promising agent for the treatment of Alzheimer's disease due to its potent acetylcholinesterase (AChE) activity, as well its efficacy in the treatment of memory of aged patients. Thus, the effects of two Huperzia species of habitats in Brazil (H. quadrifariata and H. reflexa) with described in vitro AChE inhibition activities were studied and their effects on mice brain AChE inhibition were determined after a single intraperitoneal (i.p.) injection. The alkaloid extracts were administered to mice in various doses (10, 1 and 0.5 mg/kg) and acetylcholinesterase activity was measured post mortem in two brain areas using the Ellman's colorimetric method. The AChE activity was found to be significantly reduced in both the cortex and hippocampus, although this activity was less potent than that of reference inhibitor huperzine A (0.5 mg/kg). Thus, it appears that H. quadrifariata and H. reflexa alkaloid extracts, shown to inhibit acetylcholinesterase in vitro, also have very potent in vivo effects, suggesting that the Huperzia species may still constitute a promising source of compounds with pharmaceutical interest for Alzheimer's disease.