Artículos de revistas
Automated quantification of protein periodic nanostructures in fluorescence nanoscopy images: Abundance and regularity of neuronal spectrin membrane-associated skeleton
Fecha
2017-12Registro en:
Barabas, Federico Martín; Masullo, Luciano Andrés; Bordenave, Martín Diego; Giusti, Sebastian Alejandro; Unsain, Nicolas; et al.; Automated quantification of protein periodic nanostructures in fluorescence nanoscopy images: Abundance and regularity of neuronal spectrin membrane-associated skeleton; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-10
0068-1261
2045-2322
CONICET Digital
CONICET
Autor
Barabas, Federico Martín
Masullo, Luciano Andrés
Bordenave, Martín Diego
Giusti, Sebastian Alejandro
Unsain, Nicolas
Refojo, Damian
Caceres, Alfredo Oscar
Stefani, Fernando Daniel
Resumen
Fluorescence nanoscopy imaging permits the observation of periodic supramolecular protein structures in their natural environment, as well as the unveiling of previously unknown protein periodic structures. Deciphering the biological functions of such protein nanostructures requires systematic and quantitative analysis of large number of images under different experimental conditions and specific stimuli. Here we present a method and an open source software for the automated quantification of protein periodic structures in super-resolved images. Its performance is demonstrated by analyzing the abundance and regularity of the spectrin membrane-associated periodic skeleton (MPS) in hippocampal neurons of 2 to 40 days in vitro, imaged by STED and STORM nanoscopy. The automated analysis reveals that both the abundance and the regularity of the MPS increase over time and reach maximum plateau values after 14 DIV. A detailed analysis of the distributions of correlation coefficients provides indication of dynamical assembly and disassembly of the MPS.