dc.creator | Gonzalez Deniselle, Maria Claudia | |
dc.creator | Garay, Laura Ines | |
dc.creator | López, Juan José | |
dc.creator | Gonzalez, Susana Laura | |
dc.creator | Mougel, Analía | |
dc.creator | Guennoun, Rachida | |
dc.creator | Schumacher, Michael | |
dc.creator | de Nicola, Alejandro Federico | |
dc.date.accessioned | 2017-11-26T21:41:26Z | |
dc.date.accessioned | 2018-11-06T14:58:32Z | |
dc.date.available | 2017-11-26T21:41:26Z | |
dc.date.available | 2018-11-06T14:58:32Z | |
dc.date.created | 2017-11-26T21:41:26Z | |
dc.date.issued | 2004-12 | |
dc.identifier | Gonzalez Deniselle, Maria Claudia; Garay, Laura Ines; López, Juan José; Gonzalez, Susana Laura; Mougel, Analía; et al.; Progesterone treatment reduces NADPH-diaphorase/Nitric oxide synthase in Wobbler mouse motoneuron disease; Elsevier Science; Brain Research; 1014; 1-2; 12-2004; 71-79 | |
dc.identifier | 0006-8993 | |
dc.identifier | http://hdl.handle.net/11336/29138 | |
dc.identifier | 1872-6240 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1892331 | |
dc.description.abstract | Previous work demonstrated that progesterone (PROG) treatment attenuates morphological, molecular and functional abnormalities in the spinal cord of the Wobbler (Wr) mouse, a genetic model of motoneuron degeneration. Wr mice show a marked up-regulation of the nitric oxide synthesizing enzyme (NOS). Since nitric oxide is a highly reactive species, it may play a role in neuropathology of Wr mice. We now studied if PROG neuroprotection involved changes of NOS activity in motoneurons and astrocytes, determined by the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPHD) histochemical reaction. Two and four-month-old Wr mice at the progressive and stabilization stages of the disease, respectively, and their age-matched controls were left untreated or received a single 20-mg PROG pellet for 18 days. PROG reduced the high number of NADPHD-active motoneurons and white matter astrocytes in 2-month-old Wr mice but was unable to change the low number of NADPHD-active motoneurons in 4-month-old Wr mice or astrocytes in this age group. A large number of motoneurons in 2-month-old Wr mice showed a vacuolated phenotype, which was significantly reverted by PROG treatment. In summary, PROG treatment during the early symptomatic stage of the disease caused a significant reduction of NADPHD-active motoneurons and astrocytes and also reduced vacuolated degenerating cells, suggesting that blockade of NO synthesis and oxidative damage may contribute to steroid neuroprotection. | |
dc.language | eng | |
dc.publisher | Elsevier Science | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006899304005566?via%3Dihub | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.brainres.2004.04.004 | |
dc.relation | info:eu-repo/semantics/altIdentifier/pmid/15212993 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | PROGESTERONE | |
dc.subject | WOBBLER | |
dc.subject | NITRIC OXIDE SYNTHASE | |
dc.subject | NADPH-DIAPHORASE | |
dc.title | Progesterone treatment reduces NADPH-diaphorase/Nitric oxide synthase in Wobbler mouse motoneuron disease | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |