dc.creatorGonzalez Deniselle, Maria Claudia
dc.creatorGaray, Laura Ines
dc.creatorLópez, Juan José
dc.creatorGonzalez, Susana Laura
dc.creatorMougel, Analía
dc.creatorGuennoun, Rachida
dc.creatorSchumacher, Michael
dc.creatorde Nicola, Alejandro Federico
dc.date.accessioned2017-11-26T21:41:26Z
dc.date.accessioned2018-11-06T14:58:32Z
dc.date.available2017-11-26T21:41:26Z
dc.date.available2018-11-06T14:58:32Z
dc.date.created2017-11-26T21:41:26Z
dc.date.issued2004-12
dc.identifierGonzalez Deniselle, Maria Claudia; Garay, Laura Ines; López, Juan José; Gonzalez, Susana Laura; Mougel, Analía; et al.; Progesterone treatment reduces NADPH-diaphorase/Nitric oxide synthase in Wobbler mouse motoneuron disease; Elsevier Science; Brain Research; 1014; 1-2; 12-2004; 71-79
dc.identifier0006-8993
dc.identifierhttp://hdl.handle.net/11336/29138
dc.identifier1872-6240
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1892331
dc.description.abstractPrevious work demonstrated that progesterone (PROG) treatment attenuates morphological, molecular and functional abnormalities in the spinal cord of the Wobbler (Wr) mouse, a genetic model of motoneuron degeneration. Wr mice show a marked up-regulation of the nitric oxide synthesizing enzyme (NOS). Since nitric oxide is a highly reactive species, it may play a role in neuropathology of Wr mice. We now studied if PROG neuroprotection involved changes of NOS activity in motoneurons and astrocytes, determined by the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPHD) histochemical reaction. Two and four-month-old Wr mice at the progressive and stabilization stages of the disease, respectively, and their age-matched controls were left untreated or received a single 20-mg PROG pellet for 18 days. PROG reduced the high number of NADPHD-active motoneurons and white matter astrocytes in 2-month-old Wr mice but was unable to change the low number of NADPHD-active motoneurons in 4-month-old Wr mice or astrocytes in this age group. A large number of motoneurons in 2-month-old Wr mice showed a vacuolated phenotype, which was significantly reverted by PROG treatment. In summary, PROG treatment during the early symptomatic stage of the disease caused a significant reduction of NADPHD-active motoneurons and astrocytes and also reduced vacuolated degenerating cells, suggesting that blockade of NO synthesis and oxidative damage may contribute to steroid neuroprotection.
dc.languageeng
dc.publisherElsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006899304005566?via%3Dihub
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.brainres.2004.04.004
dc.relationinfo:eu-repo/semantics/altIdentifier/pmid/15212993
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectPROGESTERONE
dc.subjectWOBBLER
dc.subjectNITRIC OXIDE SYNTHASE
dc.subjectNADPH-DIAPHORASE
dc.titleProgesterone treatment reduces NADPH-diaphorase/Nitric oxide synthase in Wobbler mouse motoneuron disease
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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