info:eu-repo/semantics/article
Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants
Fecha
2005-03Registro en:
Sun, Fei; Bahat, Anat; Gakamsky, Anna; Girsh, Eliezer; Katz, Nathan; et al.; Human sperm chemotaxis: Both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants; Oxford University Press; Human Reproduction; 20; 3; 3-2005; 761-767
0268-1161
1460-2350
CONICET Digital
CONICET
Autor
Sun, Fei
Bahat, Anat
Gakamsky, Anna
Girsh, Eliezer
Katz, Nathan
Giojalas, Laura Cecilia
Tur-Kaspa, Ilan
Eisenbach, Michael
Resumen
Background: Human sperm chemotaxis to pre-ovulatory follicular fluid is well established in vitro. However, it is not known whether the female's oocyte-cumulus complex secretes sperm chemoattractants subsequent to ovulation (for enabling sperm chemotaxis within the Fallopian tube) and, if so, which of these cell types - the oocyte or the cumulus oophorus - is the physiological origin of the secreted chemoattractant. Methods: By employing a directionality-based chemotaxis assay, we examined whether media conditioned with either individual, mature (metaphase II) human oocytes or the surrounding cumulus cells attract human sperm by chemotaxis. Results: We observed sperm chemotaxis to each of these media, suggesting that both the oocyte and the cumulus cells secrete sperm chemoattractants. Conclusions: These observations suggest that sperm chemoattractants are secreted not only prior to ovulation within the follicle, as earlier studies have demonstrated, but also after oocyte maturation outside the follicle, and that there are two chemoattractant origins: the mature oocyte and the surrounding cumulus cells.