dc.creator | Gandini, Norberto Ariel | |
dc.creator | Fermento, María Eugenia | |
dc.creator | Salomón, Débora Gisele | |
dc.creator | Obiol, Diego Javier | |
dc.creator | Andrés, Nancy Carolina | |
dc.creator | Zenklusen, Jean C. | |
dc.creator | Arevalo, Julian | |
dc.creator | Blasco, Jorge | |
dc.creator | Lopez, Alejandro | |
dc.creator | Facchinetti, Maria Marta | |
dc.creator | Curino, Alejandro Carlos | |
dc.date.accessioned | 2016-07-19T20:33:55Z | |
dc.date.available | 2016-07-19T20:33:55Z | |
dc.date.created | 2016-07-19T20:33:55Z | |
dc.date.issued | 2013-11 | |
dc.identifier | Gandini, Norberto Ariel; Fermento, María Eugenia; Salomón, Débora Gisele; Obiol, Diego Javier; Andrés, Nancy Carolina; et al.; Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma; Springer; Tumor Biology; 35; 3; 11-2013; 2803-2815 | |
dc.identifier | 1010-4283 | |
dc.identifier | http://hdl.handle.net/11336/6617 | |
dc.description.abstract | In human glioma tumors, heme oxygenase-1 (HO-1) has been shown to be upregulated both when compared with normal brain tissues and also during oligodendroglioma progression. The cell types that express HO-1 have been shown to be mainly macrophages/microglia and T cells. However, many other reports also demonstrated that cell lines derived from glioma tumors and astrocytes express HO-1 after the occurrence of a wide variety of cell injuries and stressors. In addition, the significance of HO-1 upregulation in glioma had not, so far, been addressed. We therefore aimed at investigating the expression and significance of HO-1 in human glial tumors. For this purpose, we performed a wide screening of HO-1 expression in gliomas by using tissue microarrays containing astrocytomas, oligodendrogliomas, mixed tumors, and normal brain tissues. We subsequently correlated protein expression with patient clinicopathological data. We found differences in HO-1 positivity rates between non-malignant brain (22 %) and gliomas (54 %, p = 0.01). HO-1 was expressed by tumor cells and showed cytoplasmic localization, although 19 % of tumor samples also depicted nuclear staining. Importantly, a significant decrease in the overall survival time of grade II and III astrocytoma patients with HO-1 expression was observed. This result was validated at the mRNA level in a cohort of 105 samples. However, no association of HO-1 nuclear localization with patient survival was detected. In vitro experiments aimed at investigating the role of HO-1 in glioma progression showed that HO-1 modulates glioma cell proliferation, but has no effects on cellular migration. In conclusion, our results corroborate the higher frequency of HO-1 protein expression in gliomas than in normal brain, demonstrate that HO-1 is expressed by glial malignant cells, and show an association of HO-1 expression with patients’ shorter survival time. | |
dc.language | eng | |
dc.publisher | Springer | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/10.1007/s13277-013-1373-z | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s13277-013-1373-z | |
dc.relation | info:eu-repo/semantics/altIdentifier/pmid/24234335 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs13277-013-1373-z | |
dc.rights | https://creativecommons.org/licenses/by-nc/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Heme Oxygenase-1 | |
dc.subject | Glioma | |
dc.subject | Astrocytoma | |
dc.subject | Survival | |
dc.subject | Tissue Microarray | |
dc.title | Heme oxygenase-1 expression in human gliomas and its correlation with poor prognosis in patients with astrocytoma | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |