dc.creatorKoo, Sue Jie
dc.creatorSpratt, Heidi M.
dc.creatorSoman, Kizhake V.
dc.creatorStafford, Susan
dc.creatorGupta, Shivali
dc.creatorPetersen, John R.
dc.creatorZago, María Paola
dc.creatorKuyumcu Martinez, Muge N.
dc.creatorBrasier, Allan R.
dc.creatorWiktorowicz, John E.
dc.creatorGarg, Nisha Jain
dc.date.accessioned2018-02-27T18:32:09Z
dc.date.available2018-02-27T18:32:09Z
dc.date.created2018-02-27T18:32:09Z
dc.date.issued2016
dc.identifierKoo, Sue Jie; Spratt, Heidi M.; Soman, Kizhake V.; Stafford, Susan; Gupta, Shivali; et al.; S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure; Hindawi Publishing Corporation; International Journal of Proteomics; 2016; 2016; 1-19; 1384523
dc.identifier2090-2174
dc.identifierhttp://hdl.handle.net/11336/37293
dc.identifierCONICET Digital
dc.identifierCONICET
dc.description.abstractNitric oxide (NO) protects the heart against ischemic injury; however, NO- and superoxide-dependent S-nitrosylation (S-NO) of cysteines can affect function of target proteins and play a role in disease outcome. We employed 2D-GE with thiol-labeling FL-maleimide dye and MALDI-TOF MS/MS to capture the quantitative changes in abundance and S-NO proteome of HF patients (versus healthy controls, n = 30/group). We identified 93 differentially abundant (59-increased/34-decreased) and 111 S-NO-modified (63-increased/48-decreased) protein spots, respectively, in HF subjects (versus controls, fold-change | ≥1.5|, p ≤ 0.05). Ingenuity pathway analysis of proteome datasets suggested that the pathways involved in phagocytes´ migration, free radical production, and cell death were activated and fatty acid metabolism was decreased in HF subjects. Multivariate adaptive regression splines modeling of datasets identified a panel of proteins that will provide >90% prediction success in classifying HF subjects. Proteomic profiling identified ATP-synthase, thrombospondin-1 (THBS1), and vinculin (VCL) as top differentially abundant and S-NO-modified proteins, and these proteins were verified by Western blotting and ELISA in different set of HF subjects. We conclude that differential abundance and S-NO modification of proteins serve as a mechanism in regulating cell viability and free radical production, and THBS1 and VCL evaluation will potentially be useful in the prediction of heart failure.
dc.languageeng
dc.publisherHindawi Publishing Corporation
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1155/2016/1384523
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ijpro/2016/1384523/
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectS-NITROSYLATION
dc.subjectPROTEOMIC PROFILING
dc.subjectHEART FAILURE
dc.titleS-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failure
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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