info:eu-repo/semantics/article
Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species
Fecha
2011-10Registro en:
Konrath, Eduardo Luis; Ortega, María Gabriela; Bordignon, Sergio de Loreto; Apel, Miriam Anders; Henriques, Amalia Teresinha; et al.; Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species; Taylor & Francis Ltd; Journal Of Enzyme Inhibition And Medicinal Chemistry; 28; 1; 10-2011; 218-222
1475-6366
1475-6374
CONICET Digital
CONICET
Autor
Konrath, Eduardo Luis
Ortega, María Gabriela
Bordignon, Sergio de Loreto
Apel, Miriam Anders
Henriques, Amalia Teresinha
Cabrera, Jose Luis
Resumen
The alkaloid extracts of four Huperzia and one Lycopodiella species, from Brazilian habitats, were tested for their in vitro anticholinesterase activities. IC(50) values showed a potent acetylcholinesterase inhibition for H. reflexa (0.11 ± 0.05 μg/mL), followed by H. quadrifariata (2.0 ± 0.3 μg/mL), H. acerosa (5.5 ± 0.9 μg/mL), H. heterocarpon (25.6 ± 2.7 μg/mL) and L. cernua (42.6 ± 1.5 μg/mL). A lower inhibition of butyrylcholinesterase was observed for all species with the exception of H. heterocarpon (8.3 ± 0.9 μg/mL), whose alkaloid extract presented a selectivity for pseudocholinesterase. Moreover, the chemical study of the bioactive extracts performed by GC-MS, revealed the presence of a number of Lycopodium alkaloids belonging to the lycopodane, flabellidane and cernuane groups. Surprisingly, the potent acetylcholinesterase inhibitors huperzines A and B were not detected in the extracts, suggesting that other alkaloids may be responsible for such an effect.