Artículos de revistas
In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound
Fecha
2014-03Registro en:
Rizzitelli, Silvia; Giustetto, Pierangela; Boffa, Cinzia; Delli Castelli, Daniela; Cutrin, Juan Carlos; et al.; In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound; Elsevier Science; Nanomedicine-nanotechnology Biology And Medicine; 10; 5; 3-2014; 901-904
1549-9634
Autor
Rizzitelli, Silvia
Giustetto, Pierangela
Boffa, Cinzia
Delli Castelli, Daniela
Cutrin, Juan Carlos
Aime, Silvio
Terreno, Enzo
Resumen
The work aimed at developing a MRI-guided protocol for the visualization of the release of material entrapped in liposomes stimulated by the local application of pulsed low-intensity non-focused ultrasound (pLINFU). The task was achieved by formulating liposomes filled up with the clinically approved paramagnetic agent gadoteridol, because the release of the agent from the nanovesicles is accompanied by a significant MRI signal enhancement. The protocol was validated in vivo on mice-bearing subcutaneous syngeneic B16 melanoma and i.v. injected with the paramagnetic liposomes. Upon exposing tumor to pLINFU (3 MHz, insonation time 2 min, duty cycle 50%) few minutes after liposomes injection, a signal enhancement of ca. 35% was detected. The effective release of the agent was confirmed by the strong enhancement measured in kidneys calyx and bladder due to the rapid renal excretion of the agent released in the tumor.From the Clinical EditorIn this paper, a pulsed low-intensity non-focused ultrasound-based technique was used to release a paramagnetic MRI contrast agent from liposomes, demonstrating the feasibility of this triggered release system in a mouse melanoma model for future research applications.