Artículos de revistas
TRPC3 participates in angiotensin II type 1 receptor-dependent stress-induced slow increase in intracellular Ca2+ concentration in mouse cardiomyocytes
Fecha
2017-01Registro en:
Yamaguchi, Yohei; Iribe, Gentaro; Kaneko, Toshiyuki; Takahashi, Ken; Numaga-Tomita, Takuro; et al.; TRPC3 participates in angiotensin II type 1 receptor-dependent stress-induced slow increase in intracellular Ca2+ concentration in mouse cardiomyocytes; Springer Tokyo; Journal Of Physiological Sciences; 68; 2; 1-2017; 153-164
1880-6546
CONICET Digital
CONICET
Autor
Yamaguchi, Yohei
Iribe, Gentaro
Kaneko, Toshiyuki
Takahashi, Ken
Numaga-Tomita, Takuro
Nishida, Motohiro
Birnbaumer, Lutz
Naruse, Keiji
Resumen
When a cardiac muscle is held in a stretched position, its [Ca2+] transient increases slowly over several minutes in a process known as stress-induced slow increase in intracellular Ca2+ concentration ([Ca2+]i) (SSC). Transient receptor potential canonical (TRPC) 3 forms a non-selective cation channel regulated by the angiotensin II type 1 receptor (AT1R). In this study, we investigated the role of TRPC3 in the SSC. Isolated mouse ventricular myocytes were electrically stimulated and subjected to sustained stretch. An AT1R blocker, a phospholipase C inhibitor, and a TRPC3 inhibitor suppressed the SSC. These inhibitors also abolished the observed SSC-like slow increase in [Ca2+]i induced by angiotensin II, instead of stretch. Furthermore, the SSC was not observed in TRPC3 knockout mice. Simulation and immunohistochemical studies suggest that sarcolemmal TRPC3 is responsible for the SSC. These results indicate that sarcolemmal TRPC3, regulated by AT1R, causes the SSC.