info:eu-repo/semantics/article
Carbon nanotubes non-covalently functionalized with cytochrome c: A new bioanalytical platform for building bienzymatic biosensors
Fecha
2016-09Registro en:
Eguilaz Rubio, Marcos; Venegas, Constanza J.; Gutierrez, Alejandro; Rivas, Gustavo Adolfo; Bollo, Soledad; Carbon nanotubes non-covalently functionalized with cytochrome c: A new bioanalytical platform for building bienzymatic biosensors; Elsevier Science; Microchemical Journal; 128; 9-2016; 161-165
0026-265X
CONICET Digital
CONICET
Autor
Eguilaz Rubio, Marcos
Venegas, Constanza J.
Gutierrez, Alejandro
Rivas, Gustavo Adolfo
Bollo, Soledad
Resumen
We report for the first time the use of glassy carbon electrodes (GCE) modified by drop-coating with multi-walled carbon nanotubes (MWCNTs) dispersed in cytochrome c as platform for the construction of bienzymatic biosensors, using the glycoenzyme glucose oxidase (GOx) as model. The sensitivity of the resulting biosensor at -0.050 V towards glucose using hydroquinone (H2Q) as redox mediator was (96 ± 2) μA M-1, with a linear range between 1.00 × 10-4 M and 1.00 × 10-3 M and a detection limit of 8 μM. The biosensor demonstrated to be highly reproducible with RSD. values of 6.1% for five different bioelectrodes. The combination of the excellent dispersing properties of Cyt c, the stability of MWCNTs-Cyt c dispersion and the intimate interaction between Cyt c, MWCNTs and GOx, allowed the construction of an efficient glucose biosensor successfully used to determine glucose in commercial beverages.