dc.creator | Pellegrotti, Jesica Vanesa | |
dc.creator | Acuña, Guillermo | |
dc.creator | Puchkova, Anastasiya | |
dc.creator | Holzmeister, Phil | |
dc.creator | Gietl, Andreas | |
dc.creator | Lalkens, Birka | |
dc.creator | Stefani, Fernando Daniel | |
dc.creator | Tinnefeld, Philip | |
dc.date.accessioned | 2016-02-10T15:51:25Z | |
dc.date.accessioned | 2018-11-06T14:30:34Z | |
dc.date.available | 2016-02-10T15:51:25Z | |
dc.date.available | 2018-11-06T14:30:34Z | |
dc.date.created | 2016-02-10T15:51:25Z | |
dc.date.issued | 2014-04 | |
dc.identifier | Pellegrotti, Jesica Vanesa; Acuña, Guillermo; Puchkova, Anastasiya; Holzmeister, Phil; Gietl, Andreas; et al.; Controlled reduction of photobleaching in DNA origami gold nanoparticle hybrids; American Chemical Society; Nano Letters; 14; 5; 4-2014; 2831-2836 | |
dc.identifier | 1530-6984 | |
dc.identifier | http://hdl.handle.net/11336/4098 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1887172 | |
dc.description.abstract | The amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles? size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching. | |
dc.language | eng | |
dc.publisher | American Chemical Society | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/nl500841n | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/DOI:10.1021/nl500841n | |
dc.relation | info:eu-repo/semantics/altIdentifier/issn/1530-6984 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | DNA SELF-ASSEMBLY | |
dc.subject | SINGLE MOLECULE | |
dc.subject | PHOTOBLEACHING | |
dc.subject | PLASMONICS | |
dc.title | Controlled reduction of photobleaching in DNA origami gold nanoparticle hybrids | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |