dc.creatorPellegrotti, Jesica Vanesa
dc.creatorAcuña, Guillermo
dc.creatorPuchkova, Anastasiya
dc.creatorHolzmeister, Phil
dc.creatorGietl, Andreas
dc.creatorLalkens, Birka
dc.creatorStefani, Fernando Daniel
dc.creatorTinnefeld, Philip
dc.date.accessioned2016-02-10T15:51:25Z
dc.date.accessioned2018-11-06T14:30:34Z
dc.date.available2016-02-10T15:51:25Z
dc.date.available2018-11-06T14:30:34Z
dc.date.created2016-02-10T15:51:25Z
dc.date.issued2014-04
dc.identifierPellegrotti, Jesica Vanesa; Acuña, Guillermo; Puchkova, Anastasiya; Holzmeister, Phil; Gietl, Andreas; et al.; Controlled reduction of photobleaching in DNA origami gold nanoparticle hybrids; American Chemical Society; Nano Letters; 14; 5; 4-2014; 2831-2836
dc.identifier1530-6984
dc.identifierhttp://hdl.handle.net/11336/4098
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1887172
dc.description.abstractThe amount of information obtainable from a fluorescence-based measurement is limited by photobleaching: Irreversible photochemical reactions either render the molecules nonfluorescent or shift their absorption and/or emission spectra outside the working range. Photobleaching is evidenced as a decrease of fluorescence intensity with time, or in the case of single molecule measurements, as an abrupt, single-step interruption of the fluorescence emission that determines the end of the experiment. Reducing photobleaching is central for improving fluorescence (functional) imaging, single molecule tracking, and fluorescence-based biosensors and assays. In this single molecule study, we use DNA self-assembly to produce hybrid nanostructures containing individual fluorophores and gold nanoparticles at a controlled separation distance of 8.5 nm. By changing the nanoparticles? size we are able to systematically increase the mean number of photons emitted by the fluorophores before photobleaching.
dc.languageeng
dc.publisherAmerican Chemical Society
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/nl500841n
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/DOI:10.1021/nl500841n
dc.relationinfo:eu-repo/semantics/altIdentifier/issn/1530-6984
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDNA SELF-ASSEMBLY
dc.subjectSINGLE MOLECULE
dc.subjectPHOTOBLEACHING
dc.subjectPLASMONICS
dc.titleControlled reduction of photobleaching in DNA origami gold nanoparticle hybrids
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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