Interaction of catalase with carrageenan applied to its recovery from murine liver

dc.creatorBelluzo, María Soledad
dc.creatorGalante, Micaela
dc.creatorPicó, Guillermo Alfredo
dc.creatorBoeris, Valeria
dc.date.accessioned2016-06-02T18:57:23Z
dc.date.accessioned2018-11-06T14:24:34Z
dc.date.available2016-06-02T18:57:23Z
dc.date.available2018-11-06T14:24:34Z
dc.date.created2016-06-02T18:57:23Z
dc.date.issued2013-06
dc.identifierBelluzo, María Soledad; Galante, Micaela; Picó, Guillermo Alfredo; Boeris, Valeria; Interaction of catalase with carrageenan applied to its recovery from murine liver; Elsevier; Separation and Purification Technology; 111; 6-2013; 125-130
dc.identifier1383-5866
dc.identifierhttp://hdl.handle.net/11336/6026
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1886036
dc.description.abstractThe complexes formation between acid and basic polyelectrolytes (carrageenan and Eudragit ® EPO) with catalase was evaluated in order to understand the mechanism of their interaction. Catalase was selected as the model enzyme since its application in several biotechnological processes. The study of this interaction had showed that the non soluble complexes take place in a pH interval which depends on the nature of the polyelectrolyte and salt presence. Carrageenan showed to be the best choice for the catalase affinity precipitation, since the amount needed for the full precipitation of the enzyme was lower, with a faster kinetic and a slight stabilization of the protein structure. After establishing the optimal conditions, we were able to purify catalase from murine liver, a natural source, with a purification factor of 6.4 and a yield of 19%, using only one step in the enzyme purification process.
dc.languageeng
dc.publisherElsevier
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383586613001652
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.seppur.2013.03.027
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.seppur.2013.03.027
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectCatalase
dc.subjectCarrageenan
dc.subjectEudragit EPO
dc.subjectAffinity precipitation
dc.subjectPurification
dc.titleInteraction of catalase with carrageenan applied to its recovery from murine liver
dc.typeArtículos de revistas
dc.typeArtículos de revistas
dc.typeArtículos de revistas


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